We narrowed to 1,322 results for: pET28a

1. pET28a-SrtAdelta59

   Plasmid

   #51138

   Purpose

   expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

   Depositor

   Hidde Ploegh

   Article

   Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

   Insert

   S.aureus SrtA delta 59
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pET28a hMDH2

   Plasmid

   #204230

   Purpose

   Bacterial expression of mitochondrial human malate dehydrogenase 2 (hMDH2) transcript variant 1; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   hMDH2 (MDH2 Human)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a MDHC_YEAST

   Plasmid

   #204232

   Purpose

   Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHC_YEAST (MDH2 Budding Yeast)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a-cMSPdH5

   Plasmid

   #199771

   Purpose

   Expresses split-intein fused MSPdH5 for circularization during expression (external His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH5
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   external His-Tags flanking the C and N termini

   Promoter

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Use

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Promoter

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a MDHM_YEAST

   Plasmid

   #204231

   Purpose

   Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHM_YEAST (MDH1 Budding Yeast)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-KIRIN1

   Plasmid

   #107414

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a MISTIC

   Plasmid

   #85999

   Purpose

   bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

   Depositor

   Wolfram Antonin

   Article

   Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

   Insert

   N/A
   

   Use

   Tags

   HIS6 and MISTIC

   Expression

   Bacterial

   Mutation

   codon optimzed for bacterial expression

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-SnoopCatcher

   Plasmid

   #72322

   Purpose

   Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a-SnoopCatcher
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed G842T and D848G of S. pneumoniae adhesin …

   Promoter

   T7

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-MSP1D1delltaH5

    Plasmid

    #71714

    Purpose

    Membrane scaffold protein (MSP), helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET28a-VcRibB

    Plasmid

    #187977

    Purpose

    Expresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio cholerae

    Depositor

    Audrey Lamb

    Article

    Kenjic et al J Am Chem Soc. 2022 Jul 20;144(28):12769

    Insert

    VcRibB
    

    Use

    Tags

    Hexahistag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-At-TRL

    Plasmid

    #32242

    Depositor

    Stan Fields, Jay Hesselberth

    Article

    Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

    Insert

    A. thaliana tRNA ligase (RNL Mustard Weed)
    

    Use

    Tags

    6xHistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7 RNA Polmyerase

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-cerebody

    Plasmid

    #236738

    Purpose

    Protein overexpression of cerebody

    Depositor

    Christina Woo

    Article

    Lloyd et al Cell Chem Biol. 2025 Aug 5:S2451-9456(25

    Insert

    cerebody
    

    Use

    Tags

    His, HA, FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a MDHP_YEAST

    Plasmid

    #204233

    Purpose

    Bacterial expression of yeast peroxisomal malate dehydrogenase ; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDHP_YEAST (MDH3 Budding Yeast)
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    The last 3 C-terminal residues (SKL) are the pero…

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-HsSENP1

    Plasmid

    #71465

    Purpose

    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-hDcp2

    Plasmid

    #72214

    Purpose

    bacterial expression of His-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pET28A-Rab8A

    Plasmid

    #228880

    Purpose

    Expression of His-tagged Rab8A in E. coli

    Depositor

    Sebastian Mathea

    Article

    Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

    Insert

    RAB8A (RAB8A Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    residues 1-6 and 177-207 missing

    Promoter

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-SpyTagMBP

    Plasmid

    #35050

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyTagMBP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Simple peptide tag added to the N-terminus of MBP…

    Promoter

    T7 promoter

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28a His6HuR

    Plasmid

    #135943

    Purpose

    Expresses HuR in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28a-ec_bPAC

    Plasmid

    #28135

    Purpose

    Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

    Depositor

    Peter Hegemann

    Article

    Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

    Insert

    E.coli adapted photoactivated adenylyl cyclase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28a-SUPREM

    Plasmid

    #205023

    Purpose

    Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

    Depositor

    Paola Laurino

    Article

    Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

    Insert

    SUPer Rna Ecogii Methyltransferase
    

    Use

    Tags

    His6x tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET28a(+)-SUMO-A'

    Plasmid

    #209115

    Purpose

    Bacterial expression of CC-GEMS ligand SUMO-A'

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET28a-HsSENP2

    Plasmid

    #71466

    Purpose

    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Gene optimised for E. coli expression (Genscript)

    Promoter

    T7

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pET28a-PTBP1CCtoSS

    Plasmid

    #89157

    Purpose

    Recombinant expression of PTBP1 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-MSP1D1deltaH4H5

    Plasmid

    #71716

    Purpose

    Membrane scaffold protein (MSP), helix 4+ helix 5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET28a-SET

    Plasmid

    #234708

    Purpose

    expresses His-tagged human SET in E coli

    Depositor

    Lienhard Schmitz

    Article

    Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

    Insert

    SET (SET Human)
    

    Use

    Tags

    (His)6

    Expression

    Bacterial

    Mutation

    Promoter

    lacI

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Nampt-PET28a

    Plasmid

    #25630

    Depositor

    Cynthia Wolberger

    Article

    Wang et al Nat Struct Mol Biol. 2006 Jul . 13(7):66

    Insert

    Nampt (Nampt Mouse)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only