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Plasmid#42495PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertENDOGL1A
UseTagsHis6ExpressionBacterialMutationSee CommentsPromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
Donor_C_FH_VIR
Plasmid#186660PurposeDonor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_N_FH_Shu
Plasmid#186655PurposeDonor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_C_FH_Nbr
Plasmid#186662PurposeDonor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.DepositorInsertOSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDonor_N_FH_GLE
Plasmid#186657PurposeDonor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_N_FH_Piwi
Plasmid#186651PurposeDonor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.DepositorInsertOSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Neo
Plasmid#186672PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Neomycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Hygro
Plasmid#186668PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Hygromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.HXGPRT
Plasmid#83128PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsYFPExpressionMutationPromoterEndogenous GeneAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_Bless
Plasmid#207255PurposeMammalian expression of Baboon Endogenous Retrovirus env proteinDepositorInsertBaboonEndogenousRetrovirus_Rless_env
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUS_Halo_N_allele_400
Plasmid#161767PurposeDonor vector for endogenous tagging of human FUS at the N-terminus with halotagInserthalotag (FUS Human)
UseDonor vector for endogenous taggingTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUS_Halo_C_allele_400
Plasmid#161768PurposeDonor vector for endogenous tagging of human FUS at the C-terminus with halotagInserthalotag (FUS Human)
UseDonor vector for endogenous taggingTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-GINIP-Nluc
Plasmid#223556PurposePlasmid expressing GINIP-Nluc in mammalian cellsDepositorInserthuman GINIP - linker(GGGS) - Nluc
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
YFP-CAAX
Plasmid#223558PurposePlasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation boxDepositorInsertYFP(Venus) - KRas4b
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
PUC19_C_tag
Plasmid#186659PurposeEmpty donor plasmid for C-terminal tag of 3xFlag-3xHA.DepositorInsertC-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV-14-GINIP-Nluc W139A
Plasmid#223557PurposePlasmid expressing GINIP-Nluc W139A in mammalian cellsDepositorInserthuman GINIP W139A - linker(GGGS) - Nluc
UseTags3xFLAGExpressionMammalianMutationW139A mutation in GINIP sequencePromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
puc19_N_tag
Plasmid#186650PurposeEmpty donor plasmid.DepositorInsertN-terminal 3xFlag-3xHA Tag
UseTags3xFlag-3xHAExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19_Ntag_BbsI-Mammalian
Plasmid#186674PurposeN-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.DepositorInsertMammalian N-terminal Tag
UseTags3xFlag-3xHAExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc
Plasmid#223559PurposeAlias: Gαi bONE-GO. Lentiviral vector expressing GINIP-Nluc after a low efficiency IRES downstream of YFP-CAAX placed after the promotorDepositorInsertYFP(Venus) - KRas4b - IRES* - human GINIP - linker(GGGS) - Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
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piwi_sgRNA
Plasmid#186653Purposepiwi sgRNA plasmidDepositorInsertPiwi sgRNA Plasmid (piwi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nbr_C_sgRNA2
Plasmid#186664PurposeNbr C-tag sgRNA2 plasmidDepositorInsertNbr sgRNA 2 Plasmid (Nbr Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Nbr_C_sgRNA1
Plasmid#186663PurposeNbr C-tag sgRNA1 plasmidDepositorInsertNbr sgRNA 1 Plasmid (Nbr Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Cas9_nonPuro_OSS
Plasmid#186652PurposeCas9 plasmid to express the Cas9 in OSS cellsDepositorInsertOSS Cas9 Expression Plasmid
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Cas9_YTHDF2_sgRNA
Plasmid#186673PurposeYTHDF2 sgRNA plasmidDepositorInsertYTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-YFP-CAAX-IRES*-GINIP-Nluc W139A
Plasmid#223560PurposeAlias: Gαi bONE-GO W139A. Lentiviral vector expressing GINIP-Nluc W139A after a low efficiency IRES downstream of YFP-CAAX placed after the promotorDepositorInsertYFP(Venus) - KRas4b - IRES* - human GINIP W139A - linker(GGGS) - Nluc
UseLentiviralTagsExpressionMammalianMutationW139A mutation in GINIP sequencePromoterCMVAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cas9_ANKRD1_sgRNA2
Plasmid#186669PurposePlasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.DepositorInsertANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
VPS35_Halo_C_allele
Plasmid#178175PurposeDonor vector for endogenous tagging of human VPS35 at the C-terminus with halotagInsertHalotag (VPS35 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PFN1_Halo_C_allele
Plasmid#178117PurposeDonor vector for endogenous tagging of human PFN1 at the C-terminus with halotagInsertHalotag (PFN1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNCB_Halo_C_allele
Plasmid#178130PurposeDonor vector for endogenous tagging of human SNCB at the C-terminus with halotagInsertHalotag (SNCB Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
OTUD4_Halo_C_allele
Plasmid#178164PurposeDonor vector for endogenous tagging of human OTUD4 at the C-terminus with halotagInsertHalotag (OTUD4 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TREM2_Halo_N_allele
Plasmid#178122PurposeDonor vector for endogenous tagging of human TREM2 at the N-terminus with halotagInsertHalotag (TREM2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PSEN2_Halo_C_allele
Plasmid#178133PurposeDonor vector for endogenous tagging of human PSEN2 at the C-terminus with halotagInsertHalotag (PSEN2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TBK1_Halo_C_allele
Plasmid#178172PurposeDonor vector for endogenous tagging of human TBK1 at the C-terminus with halotagInsertHalotag (TBK1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SORL1_Halo_C_allele
Plasmid#178147PurposeDonor vector for endogenous tagging of human SORL1 at the C-terminus with halotagInsertHalotag (SORL1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PARK7_Halo_C_allele
Plasmid#178165PurposeDonor vector for endogenous tagging of human PARK7 at the C-terminus with halotagInsertHalotag (PARK7 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
RNF216_Halo_C_allele
Plasmid#178167PurposeDonor vector for endogenous tagging of human RNF216 at the C-terminus with halotagInsertHalotag (RNF216 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SLC1A3_Halo_C_allele
Plasmid#178170PurposeDonor vector for endogenous tagging of human SLC1A3 at the C-terminus with halotagInsertHalotag (SLC1A3 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
SERPINI1_Halo_C_allele
Plasmid#178168PurposeDonor vector for endogenous tagging of human SERPINI1 at the C-terminus with halotagInsertHalotag (SERPINI1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBQLN2_Halo_C_allele
Plasmid#178119PurposeDonor vector for endogenous tagging of human UBQLN2 at the C-terminus with halotagInsertHalotag (UBQLN2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PANK2_Halo_C_allele
Plasmid#178145PurposeDonor vector for endogenous tagging of human PANK2 at the C-terminus with halotagInsertHalotag (PANK2 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SGP11_Halo_N_allele
Plasmid#178171PurposeDonor vector for endogenous tagging of human SGP11 at the N-terminus with halotagInsertHalotag (SPG11 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
PLEKHG4_Halo_C_allele
Plasmid#178128PurposeDonor vector for endogenous tagging of human PLEKHG4 at the C-terminus with halotagInsertHalotag (PLEKHG4 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
TIA1_Halo_N_allele
Plasmid#178149PurposeDonor vector for endogenous tagging of human TIA1 at the N-terminus with halotagInsertHalotag (TIA1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
SIGMAR1_Halo_C_allele
Plasmid#178120PurposeDonor vector for endogenous tagging of human SIGMAR1 at the C-terminus with halotagInsertHalotag (SIGMAR1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only