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We narrowed to 121 results for: HBB

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Showing: 1 - 50 of 121 results

  2. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Mutation
    dead Cas9
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

    Enlarge

  3. pAAV-CMV-dSa VP64 Hbb

    Plasmid
    #99693
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99693-plasmid-map-sequence-id-194561

    Enlarge

  10. pSA128 A2UCOE-HBB_IVS2-Cas9-BFP

    Plasmid
    #249152
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-Cas9-TagBFP (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249152-plasmid-map-sequence-id-496394

    Enlarge

  11. pSA073 HBB_IVS2-Cas9-BFP

    Plasmid
    #249134
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HBB_IVS2-Cas9-TagBFP (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249134-plasmid-map-sequence-id-496493

    Enlarge

  12. pSA075 Cas9-BFP-HBB_IVS2

    Plasmid
    #249136
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 3' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    Cas9-TagBFP-HBB_IVS2 (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249136-plasmid-map-sequence-id-496444

    Enlarge

  13. pSA072 Cas9-HBB_IVS2-BFP

    Plasmid
    #249133
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron after Cas9 coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    Cas9-HBB_IVS2-TagBFP (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249133-plasmid-map-sequence-id-496443

    Enlarge

  14. pPD685 HBB_IVS2-EnAsCas12a-BFP

    Plasmid
    #249158
    Purpose
    Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HBB_IVS2-EnAsCas12a-TagBFP (HBB Human, Synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249158-plasmid-map-sequence-id-496436

    Enlarge

  15. pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP

    Plasmid
    #249160
    Purpose
    Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-EnAsCas12a-TagBFP (HBB Human, Synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249160-plasmid-map-sequence-id-496435

    Enlarge

  16. pSA074 Cas9-BFP/HBB_IVS2/BFP

    Plasmid
    #249135
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron within BFP coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    Cas9-TagBFP/HBB_IVS2/TagBFP (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249135-plasmid-map-sequence-id-496516

    Enlarge

  17. pSA076 Cas9/HBB_IVS2/Cas9-BFP

    Plasmid
    #249137
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron within Cas9 coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    Cas9/HBB_IVS2/Cas9-TagBFP (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249137-plasmid-map-sequence-id-496396

    Enlarge

  20. pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP

    Plasmid
    #249156
    Purpose
    Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-mTagBFP2 (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249156-plasmid-map-sequence-id-496442

    Enlarge

  21. pSA144 HBB_IVS2-Cas9-BFP with core cHS4 insulators

    Plasmid
    #249154
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    HBB_IVS2-Cas9-TagBFP with core cHS4 insulators (HBB Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249154-plasmid-map-sequence-id-496518

    Enlarge

  22. pCR2.1_HBB_A

    Plasmid
    #87848
    Purpose
    pCR2.1-based plasmid holding a 328-bp fragment containing the exon-1-exon-2 splice border of aberrant HBB[IVSI-110(G>A)] human β-globin mRNA
    Depositor
    Marina Kleanthous
    Article
    Patsali et al Int J Mol Sci. 2020 Sep 11;21(18). pii:
    Insert
    Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2) (HBB Human)
    Expression
    Bacterial
    Mutation
    HBB(IVSI-110) β-thalassaemia misplicing mutation …
    Available Since
    Oct. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    87848-plasmid-map-sequence-id-313713

    Enlarge

  23. pPonA-BI-Gl NORM-LacZA TER- LacZB

    Plasmid
    #86212
    Purpose
    beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter
    Depositor
    Robert Singer
    Article
    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:
    Insert
    beta-Globin (HBB, Hbb Mouse, Human)
    Use
    Ponasterone a inducible promoter
    Tags
    lacZA or lacZB
    Expression
    Mammalian
    Mutation
    39TER in beta Globin in MCS II
    Promoter
    Ponasterone A inducible
    Available Since
    Jan. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86212-plasmid-map-sequence-id-169958

    Enlarge

  36. MLM3747

    Plasmid
    #49965
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-6 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. MLM3739

    Plasmid
    #49959
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-4 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. MLM3743

    Plasmid
    #49962
    Purpose
    Expresses a mutant TALE-TET1CD (inactive catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene for use as a control
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    3x Flag Tet1CDmut HB-5 (HBB Human)
    Use
    TALEN
    Tags
    3x FLAG
    Mutation
    catalytic domain of TET1 inactive (H1671Y and D16…
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. JA1246

    Plasmid
    #49960
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-5 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. JA1238

    Plasmid
    #49957
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-4 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

    Enlarge

  42. JA1290

    Plasmid
    #49968
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-9 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Jan. 22, 2014
    Availability
    Academic Institutions and Nonprofits only

  43. JA1261

    Plasmid
    #49963
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-6 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 31, 2013
    Availability
    Academic Institutions and Nonprofits only

  44. JA1252

    Plasmid
    #49956
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-3 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  45. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-492918

    Enlarge

  46. JA1280

    Plasmid
    #49969
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-10 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. JA1272

    Plasmid
    #49967
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-8 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  48. JA1266

    Plasmid
    #49966
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-7 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49966-plasmid-map-sequence-id-74531

    Enlarge

  49. JA1229

    Plasmid
    #49955
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-2 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. JA1217

    Plasmid
    #49954
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human beta-globin (HBB) gene and demethylate CpGs adjacent to the binding site
    Depositor
    Keith Joung
    Article
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD HB-1 (HBB Human)
    Use
    TALEN
    Promoter
    EF1a
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 50 of 121 results