We narrowed to 908 results for: pgem

1. pGEMS-II

   Plasmid

   #204765

   Purpose

   GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   EGFP-m6Alinker-DHFR
   

   Mutation

   mutated all RACs in EGFP to RGC/RCC

   Promoter

   CMV

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-Sox2

   Plasmid

   #16353

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-Oct4

   Plasmid

   #16352

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. HPRT2b-pGEM

   Plasmid

   #69539

   Purpose

   gRNA targeting human HPRT gene

   Depositor

   Hong Yan

   Article

   Liao et al Nucleic Acids Res. 2015 Jun 29. pii: gkv

   Insert

   HPRT gRNA2
   

   Expression

   Mammalian

   Available Since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEM-Nanog

   Plasmid

   #16351

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens Nanog homeobox (NANOG Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-2t

   Plasmid

   #159752

   Purpose

   CRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   sgRNA
   

   Use

   CRISPR

   Available Since

   Oct. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGEM p21

   Plasmid

   #8443

   Depositor

   Frederic Mushinski

   Article

   Huppi et al Oncogene 1994 Oct;9(10):3017-20.

   Insert

   Mouse p21 (Cdkn1a Mouse)
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pGEM-LIN28

   Plasmid

   #16350

   Depositor

   James Thomson

   Article

   Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

   Insert

   Homo sapiens lin-28 homolog (C. elegans) (LIN28A Human)
   

   Expression

   Bacterial

   Available Since

   Jan. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGem-nHyPer:cAPX

   Plasmid

   #84736

   Purpose

   Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   APX1 (APX1 Mustard Weed)
   HyPer2 targeted to nucleus
   

   Tags

   NLS-SV40

   Expression

   Plant

   Promoter

   pFMV/p35S

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGem-sHyper:sAPX

    Plasmid

    #84738

    Purpose

    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Promoter

    pFMV/p35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGem-nHyPer:sAPX

    Plasmid

    #84735

    Purpose

    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Promoter

    FMV/35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-HSPA5P

    Plasmid

    #44150

    Purpose

    a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

    Depositor

    Joan Conaway

    Article

    Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

    Insert

    HSPA5 promoter (HSPA5 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGEM-mcpGFP

    Plasmid

    #47442

    Purpose

    contains modified circularly permutated EGFP (mcpGFP)

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    mcpGFP
    

    Expression

    Bacterial

    Mutation

    M154K, V164A, S176G, D181Y, T204V, A207K, V190I

    Available Since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pGEM-gate

    Plasmid

    #113758

    Purpose

    Gateway destination vector only containing the Gateway cassette and pGEM-T Easy backbone. This can be used with Gateway-compatible Homology-Directed Repair.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    gateway cassette
    

    Use

    Gateway destination vector

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-GluCl_cryst

    Plasmid

    #31487

    Depositor

    Eric Gouaux

    Article

    Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.

    Insert

    C. elegans glutamate-gated chloride channel alpha, crystallized construct
    

    Use

    For rna synthesis for expression in x. laevis ooc…

    Tags

    8x-His

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pGEM mGata3

    Plasmid

    #25796

    Depositor

    Douglas Engel

    Article

    Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

    Insert

    Gata3 (Gata3 Mouse)
    

    Expression

    Bacterial

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pGEmCherry2

    Plasmid

    #118413

    Purpose

    Red fluorescent reporter for assessing CRISPR gRNA activity

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. mOtop3_pGEMHE

    Plasmid

    #114676

    Purpose

    This construct was used to express mouse Otop3 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 3 (Otop3 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMABC

    Plasmid

    #45398

    Depositor

    Katsu Murakami

    Article

    Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

    Inserts

    rpoA (rpoA Escherichia coli)
    rpoB (rpoB Escherichia coli)
    rpoC (rpoC Escherichia coli)
    

    Expression

    Bacterial

    Promoter

    None and T7

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. mOtop2_pGEMHE

    Plasmid

    #114675

    Purpose

    This construct was used to express mouse Otop2 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 2 (Otop2 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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