Addgene

We narrowed to 189 results for: pura

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PpurA-cfp

Plasmid

#127936

Purpose

CFP fluorescent reporter for PurA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PpurA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Article

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo

Plasmid

#212762

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-NUbo

Plasmid

#212760

Purpose

N-terminal PCR-based tagging with pCUP1-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K63R)

Plasmid

#212764

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K48R)

Plasmid

#212763

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-His7-NUbo

Plasmid

#212761

Purpose

N-terminal PCR-based tagging with pCUP1-His7-NUbo in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDZ276 (pURA MET28 PCP-2x-yeGFP)

Plasmid

#35194

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

PCP-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only

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pDZ514 pURA MET25p PP7-PS-2x-yeGFP

Plasmid

#72230

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the MET25 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

MET25

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pDZ536 pURA ADE3p PP7-PS-2x-yeGFP

Plasmid

#72234

Purpose

Uracil selection; Expression of PP7-2x-yeGFP fusion protein under the ADE3 promoter

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

PP7-PS-2x-yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

ADE3p

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pUra3-pCUP1-CUbo(K48R/K63R)

Plasmid

#212765

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF2049

Plasmid

#143866

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

PURA (PURA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pRXTN

Plasmid

#47916

Purpose

pRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.

Depositor

James Iglehart

Article

Kochupurakkal et al PLoS One. 2013 Jul 4;8(7):e68509. doi: 1

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSP64-Lifeact-GFP

Plasmid

#135601

Purpose

Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Expression

Mutation

Promoter

Unknown

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-Vasa-GFP

Plasmid

#135595

Purpose

Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Yajima et al Development. 2015 Jun 1;142(11):1960-70.

Insert

Vasa-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Expression

Mutation

Promoter

SP6

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry

Plasmid

#135598

Purpose

Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-membrane-mCherry-LOV

Plasmid

#135599

Purpose

Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

membrane-mCherry-LOV

Use

In vitro transcription (mrna synthesis)

Tags

LOV and mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-AGS-mCherry

Plasmid

#135596

Purpose

Expression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-mCherry

Use

In vitro transcription (mrna synthesis)

Tags

mCherry

Expression

Mutation

Promoter

Unknown

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-EMTB-GFP-ePDZ

Plasmid

#135600

Purpose

Expression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

EMTB-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-Lifeact-GFP-ePDZ

Plasmid

#135602

Purpose

Expression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP-ePDZ

Use

In vitro transcription (mrna synthesis)

Tags

EGFP and ePDZ

Expression

Mutation

Promoter

Unknown

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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