We narrowed to 2,493 results for: ERK

1. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Use

   Tags

   His10-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCMV5-FLAG-MKK6

   Plasmid

   #21582

   Depositor

   John Kyriakis

   Article

   Yuasa et al J Biol Chem. 1998 Aug 28. 273(35):22681-

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pLV x EF1a_EKAREN5(TA)-ires-puro

   Plasmid

   #167823

   Purpose

   control sensor for EKAREN5

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. miniTol2 x EF1a_EKAREN5(TA) + PGK-puro

   Plasmid

   #167819

   Purpose

   control sensor for EKAREN5

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. miniTol2 x EF1a_EKAREN4(TA) + PGK-blast

   Plasmid

   #167827

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. miniTol2 x EF1a_EKAREN5(TA) + PGK-blast

   Plasmid

   #167820

   Purpose

   control sensor for EKAREN5

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN5(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV x EF1a_EKAREN4(TA)-ires-puro

   Plasmid

   #167830

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

   Plasmid

   #167826

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SHIP2-ΔPR1

   Plasmid

   #214902

   Purpose

   expression of the truncated variants of the SHIP2 without first proline-rich domain

   Depositor

   Pavel Krejčí

   Article

   Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

   Insert

   human SHIP2 delta aa 123-410 (INPPL1 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   delta 123-410aa

   Promoter

   CMV

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SHIP2-C2

    Plasmid

    #214906

    Purpose

    expression of the truncated variants of the SHIP2 that retains second proline-rich domain

    Depositor

    Pavel Krejčí

    Article

    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

    Insert

    human SHIP2 aa 740-1189 (INPPL1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    delta 1-739, 1190-1258aa

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SHIP2-C1

    Plasmid

    #214905

    Purpose

    expression of the truncated variants of the SHIP2 that retains second proline-rich domain and C-terminal sterile alpha motif

    Depositor

    Pavel Krejčí

    Article

    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

    Insert

    human SHIP2 aa 740-1258 (INPPL1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    delta 1-739aa

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SHIP2-N2

    Plasmid

    #214904

    Purpose

    expression of the truncated variants of the SHIP2 that retains first proline-rich domain

    Depositor

    Pavel Krejčí

    Article

    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

    Insert

    human SHIP2 aa 123-418 (INPPL1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    delta 1-122, 419-1258aa

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SHIP2-N1

    Plasmid

    #214903

    Purpose

    expression of the truncated variants of the SHIP2 that retains only SH2 domain and first proline-rich domain

    Depositor

    Pavel Krejčí

    Article

    Fafilek et al Sci Signal. 2018 Sep 18;11(548):eaap8608

    Insert

    human SHIP2 aa 1-418 (INPPL1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    delta 419-1258aa

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV x EF1a_EKAREN4(TA)-ires-blast

    Plasmid

    #167831

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. R777-E127 Hs.MAPK3

    Plasmid

    #70411

    Purpose

    Gateway ORF clone of human MAPK3 [NM_002746.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R777-E128 Hs.MAPK3-nostop

    Plasmid

    #70412

    Purpose

    Gateway ORF clone of human MAPK3 [NM_002746.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OptoEphB2-mCherry

    Plasmid

    #92042

    Purpose

    Expresses OptoEphB2 labeled with mCherry

    Depositor

    Ji Yu

    Insert

    EPHB2 (EPHB2, CRY2 Mustard Weed, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OptoEphB2KD-mCherry

    Plasmid

    #92044

    Purpose

    Expresses kinase-dead mutant of OptoEphB2 labeled with mCherry

    Depositor

    Ji Yu

    Insert

    EPHB2 (EPHB2, CRY2 Mustard Weed, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MAPK3

    Plasmid

    #73235

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK3 (MAPK3 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00069:Pkinase 42-330

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MAP3K3

    Plasmid

    #38937

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAP3K3 (MAPK3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pDONR223_MAPK1_WT

    Plasmid

    #82145

    Purpose

    Gateway Donor vector containing MAPK1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

    Plasmid

    #167832

    Purpose

    Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    HRAS(N17) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S17N

    Promoter

    Ubc, TRE

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p663-UBC-miniTurbo-V5-MAPK4_IDG-K

    Plasmid

    #189906

    Purpose

    Gateway destination clone of MAPK4 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MAPK4 (MAPK4 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHAGE-MAPK1

    Plasmid

    #116760

    Purpose

    Lentiviral expression of MAPK1

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR223-MAPK1

    Plasmid

    #23498

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    missing first codon

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

26. pHAGE-MAPK1-E322K

    Plasmid

    #116432

    Purpose

    Lentiviral expression of MAPK1 E322K

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E322K

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. BcLOV-mCh-SOS

    Plasmid

    #208279

    Purpose

    Expresses BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    BcLOV-mCh-SOS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTriExEKAR2G2

    Plasmid

    #40175

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-smURFP-StagRFP-EKAR-EV

    Plasmid

    #173019

    Purpose

    Red/far-red FRET-based ERK activity reporter for multiplexed imaging applications.

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

    Insert

    smURFP-StagRFP-EKAR-EV
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHL-avitag3

    Plasmid

    #99847

    Purpose

    mammalian expression with secretive signal and avi tag for biotinylation

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Avi

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHL-FcHis

    Plasmid

    #99846

    Purpose

    mammalian expression with secretive signal and FC tag

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    ephrin b2 (EPHB2 Human)
    

    Use

    Tags

    Fc_His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Fus-BcLOV-mCh-SOS

    Plasmid

    #208275

    Purpose

    Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh-SOS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EKAR2G_design1_mTFP_175_Venus_173

    Plasmid

    #39830

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pTriExEKAR2G1

    Plasmid

    #40174

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EKAR2G_design1_mTFP_105_Venus_157

    Plasmid

    #39819

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

36. EKAR2G_design1_mTFP_159_Venus_173

    Plasmid

    #39825

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. EKAR2G_design1_mTFP_175_Venus_195

    Plasmid

    #39831

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

38. EKAR2G_design1_mTFP_105_Venus_195

    Plasmid

    #39821

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

39. EKAR2G_design1_mTFP_wt_Venus_wt

    Plasmid

    #39813

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. EKAR2G_design1_mTFP_227_Venus_173

    Plasmid

    #39835

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only