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Showing: 361 - 400 of 1724 results

  1. pQdCas12a.luxR(mut)-empty

    Plasmid
    #236037
    Purpose
    The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236037-plasmid-map-sequence-id-469909

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  2. pQdCas12a.luxR(mut)-sggfp(C)

    Plasmid
    #236043
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(C) expresses the dCas12a endonuclease and the sgRNA (design C) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (C) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    236043-plasmid-map-sequence-id-471082

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  3. pQdCas12a.luxR(mut)-sggfp(B)

    Plasmid
    #236041
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236041-plasmid-map-sequence-id-469952

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  4. pQdCas12a.luxR(mut)-sggfp(A)

    Plasmid
    #236039
    Purpose
    The plasmid pQdCas12a.luxR(mut)-sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    quorum sensing promoter
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236039-plasmid-map-sequence-id-469911

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  7. EKAREN5-gl

    Plasmid
    #225958
    Purpose
    The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.
    Depositor
    Toru Hiratsuka
    Article
    Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.
    Insert
    EKAREN5-gl
    Use
    Lentiviral
    Tags
    nls localization motif
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1-alpha promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    225958-plasmid-map-sequence-id-452543

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  8. R10-3 /pQE-30

    Plasmid
    #79859
    Purpose
    The first mutant of the Aequorea victoria green fluorescent protein that forms a red chromophore.
    Depositor
    Konstantin Lukyanov
    Article
    Mishin et al Biochemistry. 2008 Apr 22;47(16):4666-73
    Insert
    R10-3
    Use
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    Aequorea victoria green fluorescent protein (avGF…
    Promoter
    T5 promoter / lac operator
    Available since
    Sept. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    79859-plasmid-map-sequence-id-162057

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  10. pPEE45

    Plasmid
    #139424
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    mTurquoise1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139424-plasmid-map-sequence-id-313811

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  12. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Depositor
    Javier García-Sancho, Teresa Alonso
    Article
    Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9
    Insert
    erGAP3
    Use
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    78118-plasmid-map-sequence-id-153361

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  13. pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS

    Plasmid
    #145027
    Purpose
    Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker
    Depositor
    Dorus Gadella
    Article
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Insert
    3xnls-Tq-Ca-FLITS
    Use
    Lentiviral
    Tags
    3xnls-Tq-Ca-FLITS and H2B-Maroon
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    145027-plasmid-map-sequence-id-318435

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  14. pP150

    Plasmid
    #113201
    Purpose
    shuttle vector, N-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Dictyostelium discoideum shuttle vector, n-termin…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    113201-plasmid-map-sequence-id-217569

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  15. pPI185

    Plasmid
    #113225
    Purpose
    generic knockin vector for C-terminal tagging with mTurquoise2
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Generic knockin vector for c-terminal tagging wit…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113225-plasmid-map-sequence-id-217798

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  16. pPI193

    Plasmid
    #113224
    Purpose
    generic knockin vector for C-terminal tagging with mTurquoise2
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Generic knockin vector for c-terminal tagging wit…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113224-plasmid-map-sequence-id-217795

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  17. pPI463

    Plasmid
    #113217
    Purpose
    generic knockin vector for C-terminal tagging with mTurquoise2
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Generic knockin vector for c-terminal tagging wit…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113217-plasmid-map-sequence-id-217774

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  18. pPI182

    Plasmid
    #113226
    Purpose
    generic knockin vector for C-terminal tagging with mTurquoise2
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Generic knockin vector for c-terminal tagging wit…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113226-plasmid-map-sequence-id-217813

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  19. pPI472

    Plasmid
    #113210
    Purpose
    integrating expression vector, C-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Dictyostelium discoideum integrating expression v…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113210-plasmid-map-sequence-id-217719

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  20. pPI466

    Plasmid
    #113209
    Purpose
    integrating expression vector, N-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Dictyostelium discoideum integrating expression v…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    113209-plasmid-map-sequence-id-217682

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  21. pPI449

    Plasmid
    #113205
    Purpose
    Inducible expression vector (dox-on) N-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Dictyostelium discoideum inducible expression vec…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    113205-plasmid-map-sequence-id-217670

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  22. pPI228

    Plasmid
    #113213
    Purpose
    safe haven knock-in plasmid for the act5 locus, N-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Safe haven knock-in plasmid for the act5 locus, n…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113213-plasmid-map-sequence-id-217736

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  23. pPI229

    Plasmid
    #113214
    Purpose
    safe haven knock-in plasmid for the act5 locus, C-terminal mTurquoise2 tagging
    Depositor
    Rob Kay
    Type
    Empty backbone
    Use
    Safe haven knock-in plasmid for the act5 locus, c…
    Tags
    mTurquoise2
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    113214-plasmid-map-sequence-id-217752

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  25. THINGL pcDNA3.1

    Plasmid
    #230046
    Purpose
    Expressing the human version of TINGL biosensor for glucose (named THINGL) in mammalian cells
    Depositor
    Bas Teusink
    Article
    Botman et al bioRxiv 2024.11.29.626064
    Insert
    THINGL
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230046-plasmid-map-sequence-id-458381

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  27. THINGL-RR pcDNA3.1

    Plasmid
    #230047
    Purpose
    Expressing the human version of mScarletI-T2A-TINGL biosensor for glucose (named THINGL-RR), ratiometric variant with mScarletI.
    Depositor
    Bas Teusink
    Article
    Botman et al bioRxiv 2024.11.29.626064
    Insert
    THINGL-RR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    230047-plasmid-map-sequence-id-458390

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  28. p007ampGFP

    Plasmid
    #58535
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58535-plasmid-map-sequence-id-90574

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  30. p006-GFP-strong

    Plasmid
    #108313
    Purpose
    GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFAB4026
    Available since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    108313-plasmid-map-sequence-id-459279

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  32. p006-GFP-weak

    Plasmid
    #108314
    Purpose
    GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pFAB4282
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    108314-plasmid-map-sequence-id-220975

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  33. p006kanGFP

    Plasmid
    #58534
    Purpose
    GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Insert
    Green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T5 between two lac operators
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58534-plasmid-map-sequence-id-90573

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  34. pEXP5-NT/pT7 LasI

    Plasmid
    #193631
    Purpose
    Constitutive T7 RNAP-mediated expression of LasI 3OC12-HSL synthase for Las quorum sensing system. LasI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LasI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193631-plasmid-map-sequence-id-382852

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  35. pEXP5-NT/pT7 LuxI

    Plasmid
    #193626
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxI
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193626-plasmid-map-sequence-id-382174

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  36. pEXP5-NT/pT7 LuxR

    Plasmid
    #193625
    Purpose
    Constitutive T7 RNAP-mediated expression of LuxR transcription factor for Lux quorum sensing system. LuxR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LuxR
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193625-plasmid-map-sequence-id-388599

    Enlarge

  37. pEXP5-NT/pT7 LasR

    Plasmid
    #193629
    Purpose
    Constitutive T7 RNAP-mediated expression of LasR transcription factor for Las quorum sensing system. LasR sequence is codon optimized for E. coli.
    Depositor
    Dora Tang
    Article
    Gonzales et al ChemSystemsChem 2023
    Insert
    LasR
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNAP promoter
    Available since
    July 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    193629-plasmid-map-sequence-id-382851

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Showing: 361 - 400 of 1724 results