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Showing: 451 - 500 of 9178 results

  23. pDL14

    Plasmid
    #106901
    Purpose
    pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Inserts
    gam
    bet
    mutS
    lacI
    rhaR
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Lysine 622 was replaced by Alanine
    Promoter
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106901-plasmid-map-sequence-id-334155

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  25. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  26. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  27. pHL1746

    Plasmid
    #53034
    Purpose
    PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ycdTlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  28. pAIP4_1U8T

    Plasmid
    #234211
    Purpose
    Heterologous protein expression of flagellar motor switch protein FliM in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1U8T (fliM Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234211-plasmid-map-sequence-id-464176

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  29. pAIP4_1EW4

    Plasmid
    #234180
    Purpose
    Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EW4 (cyaY Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234180-plasmid-map-sequence-id-464145

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  30. pAIP4_3A7L

    Plasmid
    #234182
    Purpose
    Heterologous protein expression of glycine cleavage system H protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3A7L (GCV3 Escherichia coli K-12)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234182-plasmid-map-sequence-id-464147

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  31. pAIP4_3EYE

    Plasmid
    #234176
    Purpose
    Heterologous protein expression of N-acetylgalactosamine-specific enzyme IIB component of PTS in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3EYE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234176-plasmid-map-sequence-id-464141

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  32. pCASCADE-gapAP1

    Plasmid
    #87146
    Purpose
    gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87146-plasmid-map-sequence-id-174938

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  33. pJK639

    Plasmid
    #72398
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase, C-terminal His6 tag (EcPurFH6)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72398-plasmid-map-sequence-id-133991

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  35. pHL1940

    Plasmid
    #53044
    Purpose
    PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    cstA
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pHL1747

    Plasmid
    #53035
    Purpose
    PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and ydeHlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. pHL1790

    Plasmid
    #53038
    Purpose
    PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
    Depositor
    Han Lim
    Inserts
    mCherry
    csrB
    Use
    Synthetic Biology
    Tags
    glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  38. pHL1745

    Plasmid
    #53033
    Purpose
    PLtetO-1:cstAlead::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and cstAlead
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. pHL1724

    Plasmid
    #53031
    Purpose
    PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st7) and pgaAleadFull
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pHL1928

    Plasmid
    #53043
    Purpose
    PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA
    Depositor
    Han Lim
    Inserts
    mCherry
    csrA
    Use
    Synthetic Biology
    Tags
    Agn43RBS and RBS(st7)
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  41. pInt_attP1_sacB_kanR

    Plasmid
    #205301
    Purpose
    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205301-plasmid-map-sequence-id-404791

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  42. pInt_attP1_kanR

    Plasmid
    #205294
    Purpose
    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205294-plasmid-map-sequence-id-407001

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  43. pInt_attP2_kanR

    Plasmid
    #205297
    Purpose
    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#2).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    205297-plasmid-map-sequence-id-415623

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  44. pInt_attP3_chlorR

    Plasmid
    #205298
    Purpose
    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#3).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205298-plasmid-map-sequence-id-407000

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  45. pInt_attP5_ampR

    Plasmid
    #205299
    Purpose
    Integrating plasmid for ORBIT in E. coli. Orthogonal attP site (#5).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205299-plasmid-map-sequence-id-406998

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  46. pJK633

    Plasmid
    #72397
    Purpose
    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylaminoimidazole synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72397-plasmid-map-sequence-id-134629

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  47. pInt_attP1_chlorR

    Plasmid
    #205295
    Purpose
    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205295-plasmid-map-sequence-id-406999

    Enlarge

  48. pInt_attP1_ampR

    Plasmid
    #205296
    Purpose
    Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205296-plasmid-map-sequence-id-406997

    Enlarge

  49. pJK645

    Plasmid
    #71700
    Purpose
    Produces E. coli thioredoxin (EcTrxA)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71700-plasmid-map-sequence-id-131737

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  50. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Kang Zhou
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212153-plasmid-map-sequence-id-420764

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Showing: 451 - 500 of 9178 results