Addgene

Showing: 181 - 200 of 25998 results

Results per page:

Sort results by:

pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Use

Tags

6x His tag and SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mTFP1-CathespinB-6

Plasmid

#55467

Purpose

Localization: Lysosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

CathespinB

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

Plasmid

#116965

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
InterCatch-GFP

Plasmid

#186900

Purpose

Cell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

TfR-sfGFP-SpyCatcher003-sfCherry1-10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
258/-2.5CAT

Plasmid

#25431

Depositor

Charles P. Emerson Jr.

Article

Goldhamer et al Development. 1995 Mar . 121(3):637-49.

Insert

Myo D Promoter/Enhancer (MYOD1 Human)

Use

Reporter construct

Tags

CAT

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC

Plasmid

#116964

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
ACS2 Truncated

Plasmid

#13748

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 39–682

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pMXs-Ecat1

Plasmid

#13351

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 1 (2410004A20Rik Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
Pgr_beta-catenin-1

Plasmid

#107664

Purpose

RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

beta-catenin-1

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mApple-CathespinB-6

Plasmid

#54871

Purpose

Localization: Lysosomes, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

CathespinB

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pMXs-Ecat8

Plasmid

#13357

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell associated transcript 8 (Tdrd12 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
tdTomato-CathespinB-6

Plasmid

#58075

Purpose

Localization: Lysosomes, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

CathespinB (Ctsb Rat)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAT.011

Plasmid

#119555

Purpose

Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPBbsr2-SCAT3NES

Plasmid

#197350

Purpose

Encoding SCAT3NES.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

SCAT3NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC

Plasmid

#116966

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-CATULIN

Plasmid

#118979

Purpose

Expresses full length of Catulin-GFP (Mus musculus). Contains CMV and T3 promoter for mammalian expression and mRNA production.

Depositor

Tomasz Prószyński

Article

Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

Insert

Alpha Catulin (Ctnnal1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
BcatMut-Tyr654

Plasmid

#24199

Depositor

David Rimm

Insert

beta-catenin (CTNNB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Y654F

Promoter

Available since

March 23, 2010

Availability

Academic Institutions and Nonprofits only
mEmerald-CathespinB-6

Plasmid

#54024

Purpose

Localization: Lysosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

CathespinB

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEos2-CathespinB-6

Plasmid

#57354

Purpose

Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CathespinB (Ctsb Rat)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
EBFP2-CathespinB-6

Plasmid

#55236

Purpose

Localization: Lysosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Article

Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

Insert

CathespinB

Use

Tags

EBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits