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Showing: 181 - 200 of 4082 results

  1. TFORF2876

    Plasmid
    #142167
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ELF2 (ELF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    142167-plasmid-map-sequence-id-397680

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  2. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  3. pTXB1-sfGFP

    Plasmid
    #51562
    Purpose
    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
    Depositor
    Francisco Malagon, Sankar Adhya
    Insert
    superfolder GFP
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51562-plasmid-map-sequence-id-78800

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  13. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PGK/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155342-plasmid-map-sequence-id-310519

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  15. MmXYRS-4xM15

    Plasmid
    #155343
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    Humanized MmXYRS/tRNAM15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV/U6
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155343-plasmid-map-sequence-id-310521

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  18. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Use
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

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  19. DT044A

    Plasmid
    #159405
    Purpose
    Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7/LacO promoter
    Available since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    159405-plasmid-map-sequence-id-324289

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Showing: 181 - 200 of 4082 results