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pMT-HuC:Dendra2

Plasmid

#80904

Purpose

Injection into Zebrafish larvae together with Tol2-Transposase to achieve pan-neuronal expression of the photoconvertible fluorescent protein Dendra2

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

Dendra2 (elavl3 Synthetic)

Use

Zebrafish expression

Promoter

HuC

Available Since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-Kaede (Kaede-A69T)

Plasmid

#99228

Purpose

Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-Kaede

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pQE32-pr-mEosFP (mEosFP-V69T)

Plasmid

#99213

Purpose

Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEosFP

Expression

Bacterial

Mutation

monomeric variant with mutation V69T for primed c…

Promoter

T5

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.2 (mEosFP3.2-A69T)

Plasmid

#99224

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.2 (mEos3.2-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.2

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4b (mEosFP4b-V69T)

Plasmid

#99226

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4b

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4a (mEosFP4a-V69T)

Plasmid

#99225

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4a

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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LIG4 HAL-T2A-GFP-SV40pA-HAR

Plasmid

#225357

Purpose

This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the SelectRepair knockout GFP approach.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

T2A-GFP-SV40pA

Use

CRISPR

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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LIG4 HAL-T2A-HYG-SV40pA-HAR

Plasmid

#225358

Purpose

This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the hygromycin B SelectRepair knockout approach.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

T2A-HYG-SV40pA

Use

CRISPR

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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LIG4 HAL-T2A-NEO-SV40pA-HAR

Plasmid

#225359

Purpose

This plasmid serves as a template for homologous DNA recombination at the LIG4 gene to knock out the DNA ligase 4 protein using the neomycin SelectRepair knockout approach.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

T2A-NEO-SV40pA

Use

CRISPR

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pX459_LIG4_Exon3

Plasmid

#225363

Purpose

pX459 plasmid encoding the sgRNA protospacer sequence “5’-GCATAATGTCACTACAGATC-3’ to target the LIG4 gene.

Depositor

Sujatha Jagannathan

Article

Cortazar et al Methods Mol Biol. 2025;2863:397-417. doi

Insert

LIG4 (LIG4 Human)

Use

CRISPR

Promoter

U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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p55-H2B-Dendra2

Plasmid

#80609

Purpose

Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.

Depositor

Periklis Pantazis

Article

Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

Insert

H2B-Dendra2 (H2BC21 Synthetic, Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Lifect-7-pr-mEos2 (mEos2-A69T)

Plasmid

#99229

Purpose

Mammalian expression of the primed conversion capable protein pr-mEos2 (mEos2-A69T) as a Lifeact fusion.

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos2

Tags

Lifeact

Expression

Mammalian

Mutation

A69T for primed conversion applications

Promoter

CMV

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mKikGR1 (mKikGR1-V69T)

Plasmid

#99227

Purpose

Bacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mKikGR1

Expression

Bacterial

Mutation

monomeric variant with mutation V69T (Eos notatio…

Promoter

T7

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-GenEPi

Plasmid

#140236

Purpose

Mammalian expression of Piezo1-based fluorescent force sensor GenEPi

Depositor

Periklis Pantazis

Article

Yaganoglu et al Nat Commun. 2023 Jul 19;14(1):4352. doi:

Insert

GenEPi (Piezo1-based fluorescent force sensor) (PIEZO1 Human)

Tags

GCaMP 6s RS1 EF4

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA-10Xcapture-PuroR

Plasmid

#211496

Purpose

Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

Depositor

Antonella Fidanza, Lesley Forrester

Article

Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

U6-gRNA
Ef1a-PuroR

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Promoter

EF1a and U6

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-iSAM

Plasmid

#211495

Purpose

AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

Depositor

Antonella Fidanza, Lesley Forrester

Article

Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

Inserts

UniSAM insert
TET-ON
Neo Resistance
Insulator

Use

CRISPR and Synthetic Biology

Tags

T2A-mCherry Tag

Expression

Mammalian

Promoter

EF1a, NA, Splicing acceptor, and TREGV promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Tafazzin [N173B/13R]

Plasmid

#206539

Purpose

Mammalian Expression Plasmid of anti-Tafazzin (Human). Derived from hybridoma N173B/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Tafazzin (Homo sapiens) recombinant Mouse monoclonal antibody (TAFAZZIN Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PG13-mCherry

Plasmid

#195291

Purpose

mCherry reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Insert

mCherry

Expression

Mammalian

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNGN2

Plasmid

#172115

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Inserts

hNGN2 (NEUROG2 Human)
puroR-T2A-mycNLS-mTagBFP2

Tags

T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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