We narrowed to 4,673 results for: pcr

Showing: 181 - 200 of 4673 results

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pCry2PHR-mCh-Rac1

Plasmid

#42954

Purpose

Expresses optogenetically controlled Rac1

Depositor

David Schaffer

Publication

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cry2PHR-mCherry-Rac1 (RAC1 Mustard Weed, Human)

Expression

Mammalian

Promoter

CMV

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6

Plasmid

#41596

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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PCR4-Hsp68::lacZ-H11

Plasmid

#139099

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

Depositor

Len Pennacchio

Publication

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Hsp68

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRISPaint-NanoLuc-PuroR

Plasmid

#80962

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NanoLuc

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagBFP-PuroR

Plasmid

#80969

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TagBFP

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-TRP1

Plasmid

#41595

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFA6a-13Myc-kanMX6

Plasmid

#39294

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFA6a-3HA-His3MX6

Plasmid

#41600

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCR3-FLAG-Ubc13-WT

Plasmid

#43938

Depositor

Bryant Darnay

Publication

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ubiquitin-conjugating enzyme E2N (UBE2N Human)

Tags

2x FLAG

Expression

Mammalian

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-kanMX6

Plasmid

#39295

Depositor

Jurg Bahler, John Pringle

Publication

Bahler et al Yeast. 1998 Jul;14(10):943-51.

Insert

KanR

Use

Yeast genomic targeting

Tags

3HA, A. gossypii translation elongation factor 1a…

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCRISPaint-2A-TurboGFP-PEST-PuroR

Plasmid

#80956

Purpose

CRISPaint gene tagging

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TurboGFP-PEST

Use

Gene tagging

Promoter

none

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCR2.1 Clover-LMNA Donor

Plasmid

#122508

Purpose

Homology repair template for in frame (first exon) clover knock-in of human LMNA gene

Depositor

Graham Dellaire

Publication

Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

Insert

Homology Repair Template for Human LMNA (N-Terminal Clover Tag) (LMNA Human)

Use

Homology repair template plasmid (donor plasmid)

Tags

Clover

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Publication

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Available since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-13Myc-His3MX6

Plasmid

#41602

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

His3

Use

Yeast genomic targeting

Tags

13Myc, A. gossypii translation elongation factor …

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

Plasmid

#221225

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

Depositor

Fei Chen

Publication

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AID-PcrA M6-UGI-T2A-mTagBFP2

Expression

Mammalian

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-3HA-TRP1

Plasmid

#41599

Depositor

John Pringle

Publication

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA and yeast ADH1 terminator

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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PCRT7 tetR pLtetO MBP-MEK1 XX Amp

Plasmid

#53225

Purpose

human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

Depositor

Jesse Rinehart

Insert

MEK1 (MAP2K1 Human)

Use

Phosphoprotein synthesis

Tags

HIS and MBP

Expression

Bacterial

Mutation

Codon optimized for E.coli, both codons for Ser21…

Promoter

pLtetO

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCRISPaint-T2A-Gal4-3xP3-RFP

Plasmid

#127556

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4

Use

CRISPR

Expression

Insect

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-HBH-kanMX6

Plasmid

#26872

Depositor

Peter Kaiser

Publication

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HBH tag

Use

Pcr tagging yeast

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pScaffold-H1 donor

Plasmid

#118152

Purpose

PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

Depositor

Jorge Ferrer

Publication

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Type

Empty backbone

Expression

Mammalian

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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