Addgene

We narrowed to 8,310 results for: Set;

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pDD372

Plasmid

#91824

Purpose

Codon-optimized GFP donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

GFP-C1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDD377

Plasmid

#91823

Purpose

Codon-optimized mTurquoise2 donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

mTurquoise2-C1

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pND004

Plasmid

#200950

Purpose

MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.EGFP

Plasmid

#38053

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.EGFP.WPRE

Plasmid

#38055

Depositor

James Uney

Article

Glover et al Mol Ther. 2002 May;5(5 Pt 1):509-16.

Insert

EGFP

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pABCb10aa1-70-GFP

Plasmid

#19038

Depositor

Orian Shirihai

Article

Graf et al J Biol Chem. 2004 Oct 8. 279(41):42954-6

Insert

amino acids 1 - 70 of the targeting sequence of ABCb1

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
IDP205 (T7->Rci; 5bc)

Plasmid

#68834

Purpose

Expresses Rci gene (driven by the inducible T7 promoter) and 5 fragment barcode cassette in bacterial cells

Depositor

Anthony Zador

Article

Peikon et al Nucleic Acids Res. 2014;42(16):e127. doi

Inserts

Barcode cassette 5 fragments
Rci recombinase

Use

Tags

Expression

Mutation

Promoter

T7

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-M71-IRES-CreFNF

Plasmid

#15509

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

M71 targeting vector with IRES CreFNF

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pBS-M71-IRES-tauRFP2ACNf

Plasmid

#15510

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

M71 targeting vector with IRES tauRFP-RFP ACN

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pNM3810

Plasmid

#154119

Purpose

mec-4 promoter driving QF in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::QF::act-4 '3

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pYFP.LIC.HXGPRT

Plasmid

#83128

Purpose

Plasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.

Depositor

Vernon Carruthers

Article

Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:

Insert

YFP

Use

Toxoplasma gondii

Tags

YFP

Expression

Mutation

Promoter

Endogenous Gene

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSEVA12DKR-A

Plasmid

#58304

Purpose

GeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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HHV7 U69 KD

Plasmid

#26694

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

U69 (U69 Human Herpes Virus 7)

Use

Tags

HA

Expression

Mammalian

Mutation

Kinase Deficient Mutant Lys 202 mutated to Asn

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
HHV6 U69 KD

Plasmid

#26690

Depositor

Robert Kalejta

Article

Kuny et al PLoS Pathog. 2010 . 6(9):.

Insert

U69 (U69 Human Herpes Virus 6)

Use

Tags

HA

Expression

Mammalian

Mutation

Kinase Deficient Mutant: Lys 218 mutated to Met

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pSEVA12DKR-E

Plasmid

#58305

Purpose

GeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Insert

Genomic cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-OMP-CreFNF

Plasmid

#15508

Depositor

Peter Mombaerts

Article

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

OMP targeting Vector with CRE FNF

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pFF19H

Plasmid

#49783

Purpose

expresses hygromycin resistance gene in plant cells

Depositor

Joachim Messing

Article

Timmermans et al J Biotechnol. 1990 Jun;14(3-4):333-44.

Insert

HPH

Use

Plant expression

Tags

Expression

Mutation

Promoter

CaMV 35S

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
pDual_dsCas9_Puro_hs_NC_chr1

Plasmid

#214688

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

Depositor

Kevin Janes

Insert

dgRNA_Chr1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Puromycin resistance cassette has silent mutation…

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDual_dsCas9_Puro_hs_TP73

Plasmid

#214689

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human TP73

Depositor

Kevin Janes

Insert

dgRNA_TP73 (TP73 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Puromycin resistance cassette has silent mutation…

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pND005

Plasmid

#200951

Purpose

Yeast two-hybrid destination vector for Golden Gate cloning

Depositor

Timothy Whitehead

Article

Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

Type

Empty backbone

Use

Yeast two-hybrid

Tags

Expression

Mutation

Promoter

GAL4

Available since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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