We narrowed to 14,587 results for: pET
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Plasmid#218039PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00AZ-W
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00H3-S-FLAG-His
Plasmid#218038PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00H3-S
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00FH-2-FLAG-His
Plasmid#218037PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00FH-2
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+)_PelB-BN00B0-E-FLAG-His
Plasmid#218035PurposeRESOLUTE SLC binder expression plasmidDepositorInsertBN00B0-E
UseTags1xFLAG 10xHis and PelBExpressionBacterialMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His
Plasmid#218198PurposeBacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOMDepositorInsertEndoCOM
UseTags3C-CBD-HisExpressionBacterialMutationcodon optimized Ecoli, residues 19-315PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_notufA-EmEndoF2-CBD-His
Plasmid#218197PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2DepositorInsertEndoF2
UseTagsCBD-HisExpressionBacterialMutationcodon optimized Ecoli, residues 45-335PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Keyhole limpet hemocyanin [N132A/12R]
Plasmid#206532PurposeMammalian Expression Plasmid of anti-Keyhole limpet hemocyanin (Giant keyhole limpet). Derived from hybridoma N132A/12.DepositorInsertanti-Keyhole limpet hemocyanin (Megathura crenulata) recombinant Mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO-syt7 (136-403) L361C
Plasmid#213622PurposeBacterial expression of SUMO-tagged mouse syt7 (136-403) L361CDepositorInsertsyt7 (136-403) L361C (Syt7 Mouse)
UseTagsSUMOExpressionBacterialMutationC260A C275A L361CPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET 15b+His SUMO Rab12 (Q101L)
Plasmid#208371PurposeBacterial expression of His-SUMO-tagged human Rab12 (Q101L)DepositorInsertHis SUMO Rab12 (Q101L) (RAB12 Human)
UseTagsExpressionMammalianMutationQ101LPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-6xHis-TEV-Nsp8(76-198)
Plasmid#201024PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDUET-GST-AUP1 379-410:Ube2G2
Plasmid#185335PurposeUsed for co-expression of the AUP1 G2BR with UBE2G2 for crystallographyDepositorInsertAUP1 aa 379-410, Ube2G2 (AUP1 Human, Mouse)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NcQDE-2_506-938-del787-838GSG_K
Plasmid#146730PurposeBacterial Expression of NcQde-2_506-938-del787-838GSGDepositorInsertNcQde-2_506-938-del787-838GSG
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-6xhis-sumo-CeMIGA(106-496)
Plasmid#192832PurposeExpress C.elegans MIGA fragment 106-496 in bacteriaDepositorInsertMitoguardin homologue (miga-1 Nematode)
UseTags6xhis sumoExpressionBacterialMutationPromoterT7Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
human KANK2-A670V Ankyrin Repeats pET151
Plasmid#164055PurposeExpresses human KANK2 Ank domain - A670V mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mouse KANK4-Y801H Ankyrin Repeats pET151
Plasmid#164058PurposeExpresses mouse KANK4 Ank domain -Y801H mutant in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1-LanCL2-C321A/C367A-C-Ter-His
Plasmid#154188PurposeTo express LanCL2-C321A/C367A in bacterial cellsDepositorInsertLanCL2
UseTagsHexahistidineExpressionBacterialMutationCys321->Ala321; Cys367->Ala367PromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR
Plasmid#169499PurposeTier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)DepositorInsertPRPBSA-driven YPet-P2aA-PuroR
UseTagsExpressionMammalianMutationPromoterPRBSAAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1047-Tier1-PhCMV-YPet-p2A-PuroR
Plasmid#169498PurposeTier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).DepositorInsertPCMV-driven YPet-P2aA-PuroR
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His10-BTLAicd (AA190-289)-TwinStrep
Plasmid#180805PurposeExpression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrepDepositorInsertBTLAint (AA190-289)-TwinStrep (BTLA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
CEA-N domain residues 1-132 (CEACAM5)-pET30b
Plasmid#174693PurposePlasmid expressing CEA-N domainDepositorInsertCEA-N Domain
UseTagsTEV Cleavage Site and hexahistidine (HIS) tagExpressionBacterialMutationnonePromoterT7 PromoterAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis
Plasmid#176573PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184QPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q W330Y)-6xHis
Plasmid#176577PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsHexahistidine tag and mVenusExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin-FLN-ELP-His-ybbr
Plasmid#168039PurposeExpresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG-FLN-ELP-His-ybbr
Plasmid#168047PurposeExpresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminusDepositorInsertStaphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
UseTags3xELP linker, 6x Histag, ddFLN4, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)
Plasmid#91818PurposeBacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutationsDepositorInsertSPT6 (SPT6 Budding Yeast)
UseTags6xHisExpressionBacterialMutationchanged lysine 1355 and lysine 1435 to alanines; …PromoterT7Available sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-YPET-3XNLS-optoNES-WPRE
Plasmid#159947Purposeoptical regulation of nuclear exportDepositorInsertYPET-3XNLS-optoNES
UseAAVTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES-WPRE
Plasmid#159942Purposeoptical regulation of nuclear exportDepositorInsertNLS-YPET-optoNES
UseAAVTagsExpressionMammalianMutationsyntheticPromoterhSyn1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE
Plasmid#159975Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.F434L
UseAAVTagsExpressionMammalianMutationF434LPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE
Plasmid#159973Purposeoptical regulation of nuclear export with extended relaxation timeDepositorInsertNLS-YPET-optoNES.V416I
UseAAVTagsExpressionMammalianMutationV416IPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE
Plasmid#159974Purposeoptical regulation of nuclear export with shorter relaxation timeDepositorInsertNLS-YPET-optoNES.I427V
UseAAVTagsExpressionMammalianMutationI427VPromoterhSyn1Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-TBP(38Q)-IDR-mCherry
Plasmid#145283PurposeBacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion ProteinDepositorInsertTBP(38Q)-IDR (TBP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS
Plasmid#153443PurposeE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
UseTags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a human Tankyrase2 full length Y920E
Plasmid#132639Purposeexpresses human Tankyrase2 full length Y920E in E. coliDepositorInsertTankyrase2 Y920E
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79N Q109N (TAC-->TAT)
Plasmid#105738PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ79N/Q109NPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 K321D
Plasmid#89288PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Lys-321 to AspPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 R325D
Plasmid#89289PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Arg-325 to AspPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 E322A
Plasmid#87748PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Glu-322 to AlaPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 R325A
Plasmid#87749PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Arg-325 to AlaPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)-Hcp1 K321A
Plasmid#87747PurposeBacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEV and Hcp1ExpressionBacterialMutationdeleted amino acids 1-100, mutated Lys-321 to AlaPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS
Plasmid#61940PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coliDepositorUseTags6x HISExpressionBacterialMutationConstruct ends at residue 156, residues 97-100 (N…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only