We narrowed to 12,028 results for: LEA

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pBABE-Flag-Cdk9-IRES-eGFP

Plasmid

#28096

Depositor

Andrew Rice

Article

Dow et al J Cell Physiol. 2010 Jul . 224(1):84-93.

Insert

Cdk9 (CDK9 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pPY22-mNeonGreen-Hyg

Plasmid

#137082

Purpose

Codon-optimized mNeonGreen for expression in Arabidopsis thaliana and Physcomitrella patens

Depositor

Gohta Goshima

Article

Yi et al Curr Biol. 2020 Jul 20;30(14):2860-2868.

Insert

mNeonGreen-Flag

Expression

Plant

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSS146

Plasmid

#64770

Purpose

For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

Cre-EBD78

Tags

fused to the human Estrogen Binding Domain (EBD),…

Expression

Yeast

Promoter

TDH4

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-PANX1(HCVp)

Plasmid

#87698

Purpose

Express HCV protease-activatible pannexin-1 in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PANX(HCVp)

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
HOXA10-MSCV

Plasmid

#20980

Depositor

Corey Largman

Article

Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

Insert

HOXA10 (HOXA10 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pAL-hCul1-NTD

Plasmid

#29518

Depositor

Ning Zheng

Insert

CUL1 (CUL1 Human)

Tags

Thrombin Cleavage Site

Expression

Bacterial

Mutation

N-terminal 415 aa

Available Since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Article

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

Plasmid

#86413

Purpose

nuclear split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

Nop1pr-GFP11-mCherry-PUS1

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 2

Plasmid

#22210

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTet

Plasmid

#124663

Purpose

Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTet

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET His10 TEV LIC cloning vector (2B-T-10)

Plasmid

#78173

Depositor

Scott Gradia

Type

Empty backbone

Tags

10xHis and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176268

Purpose

Expression of dCas12f-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCas12f

Use

Lentiviral

Tags

HA-VPR-mCherry

Expression

Mammalian

Mutation

D326A, D510A

Promoter

PGK

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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msEGFP-PACR_pcDNA3

Plasmid

#55774

Purpose

Genetically encoded caged Ca2+

Depositor

Takeharu Nagai

Article

Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

Insert

PACR

Tags

msEGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2014

Availability

Academic Institutions and Nonprofits only
pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)

Plasmid

#29724

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mC…

Expression

Bacterial

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pMVA2RBS035

Plasmid

#121051

Purpose

Expresses recombinant MVA pathway in Escherichia coli

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

Inserts

mvaE
mvaS
mvaK1
mvaK2
mvaD
idi

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST26 Ago2

Plasmid

#59904

Purpose

Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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Nrf1-Myc pcDNA3.1(+)-C-Myc

Plasmid

#169125

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Article

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hNrf1 (NFE2L1 Human)

Expression

Mammalian

Mutation

wt

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEmCherry2

Plasmid

#118413

Purpose

Red fluorescent reporter for assessing CRISPR gRNA activity

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pKM7

Plasmid

#154164

Purpose

mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

Depositor

Kevin Maringer

Article

Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

Insert

mCherry

Expression

Insect

Promoter

IE1 promoter and hr5 enhancer from Autographa cal…

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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