We narrowed to 3,857 results for: SPL;

1. pET:CCCT:GFP

   Plasmid

   #60738

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ8454 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-WPRE

   Plasmid

   #140087

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, with EF1a driven puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3x-Flag Gli3 30R3-1

   Plasmid

   #120304

   Purpose

   Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 30C

   Depositor

   David Liu

   Article

   Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

   Insert

   30R3-1, Gli3
   

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTEI135

   Plasmid

   #126073

   Purpose

   Vector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   mitochondria Rieske iron sulphur protein (1-100 aa)
   

   Use

   Binary

   Tags

   YFP1-10

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTEI165

   Plasmid

   #126074

   Purpose

   Vector for expression of plastid targeted improved YFP1-10 (T65G,T205S, T203Y) in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   Chloroplastic FNR transit peptide
   

   Use

   Binary

   Tags

   YFP1-10 (improved)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTEI166

   Plasmid

   #126075

   Purpose

   Vector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Insert

   mitochondria Rieske iron sulphur protein (1-100 aa)
   

   Use

   Binary

   Tags

   YFP1-10 (improved)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTEI128

   Plasmid

   #126209

   Purpose

   Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   ccdB Cassette (BsaI)
   GFP11x3 (5 aa linker)
   

   Use

   Binary, module 1

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcFN-C (chick fibronectin)

   Plasmid

   #14063

   Depositor

   Richard Hynes

   Article

   Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

   Insert

   Chicken Fibronectin (FN1 Chicken)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   DraI - EcoRI fragment inserted in HincII-EcoRI si…

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3156 pBS Bad cDNA

   Plasmid

   #8752

   Depositor

   Stanley Korsmeyer

   Article

   Yang et al Cell 1995 Jan 27;80(2):285-91.

   Insert

   BAD (Bad Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pNMHCII-C2-GFP

    Plasmid

    #46043

    Depositor

    Robert Adelstein

    Article

    Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

    Insert

    nonmuscle myosin heavy chain II-C, isoform C2 (Myh14 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate early

    Available since

    July 31, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pGADT7 Hsh155 Y292C

    Plasmid

    #87291

    Purpose

    Encodes Y2H Gal4 activation domain for Hsh155 Y292C

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

    Insert

    HSH155 (HSH155 Budding Yeast)
    

    Use

    Tags

    GAL4 AD and HA

    Expression

    Bacterial and Yeast

    Mutation

    Y292C

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-PRMT9 G260E mutant

    Plasmid

    #67610

    Purpose

    bacterial expression of GST-PRMT9 G260E Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G260E double E loop mutant

    Promoter

    tac

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

13. GST-PRMT9 D258G mutant

    Plasmid

    #67609

    Purpose

    bacterial expression of GST-PRMT9 D258G Double E loop mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    D258G double E loop mutant

    Promoter

    tac

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

14. PLL7.0 tgRFP LOV

    Plasmid

    #114463

    Purpose

    Mammalian expression of tgRFP labelled LOV2

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    LOV2
    

    Use

    Tags

    tgRFP

    Expression

    Mammalian

    Mutation

    G401A(G528A), N411E(N538E) and V289I (V416I)

    Promoter

    CMV

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEX Tom20 mVenus BoPL

    Plasmid

    #114467

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoPL

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoPL
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriEx TOM20 mVenus BoLL

    Plasmid

    #114465

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoLL

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoLL
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTriEX Tom20 mVenus BoLD

    Plasmid

    #114468

    Purpose

    Mammalian expression of mitochondrially targeted, mVenus labelled BoLD

    Depositor

    Andrew Woolley

    Article

    Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

    Insert

    BoLD
    

    Use

    Tags

    HIS, TEV and FLAG and TOM20 and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEIIIB

    Plasmid

    #14060

    Depositor

    Richard Hynes

    Article

    Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

    Insert

    Rat Fibronectin EIIIB (Fn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EcoRI linkers were attached to HindIII - HinfI fr…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

19. Desmin/pBSSK+

    Plasmid

    #97207

    Purpose

    Desmin (NM_001927, nt 258 to 395) in pBSSK+

    Depositor

    Rita Shiang

    Article

    Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

    Insert

    Desmin (DES Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Prp2-pRSETA

    Plasmid

    #61042

    Purpose

    Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Prp2
    

    Use

    Tags

    Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only