We narrowed to 84,557 results for: TRI

Showing: 1981 - 2000 of 84557 results

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pEGFP-C1

Plasmid

#71378

Purpose

For analysis of PH domain localization in mammalian cells

Depositor

Mark Lemmon

Article

Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

Insert

Grp1-PH (Cyth3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Pleckstrin homology (PH) domain; aa 247-399

Promoter

CMV

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA GFP10-ZIP-GFP11

Plasmid

#182243

Purpose

Tripartite split-GFP mammalian. Express leucine zipper with GFP10 and GFP11 (self-assembing with GFP1-9)

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP10-ZIP-11

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCA24N-p50-ligase

Plasmid

#87742

Purpose

IPTG-inducible expression of p50-ligase fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and human p50/NF-kB (amino acids 40-366)

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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JD180

Plasmid

#229486

Purpose

Adenoviral helper plasmid containing inducible E2A and L4-22k/33k genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E2A, L4-22k/33k, mCitrine

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

Tre (E2A, L4-22k/33k), ETR (mCitrine)

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQmod3C-GG

Plasmid

#191350

Purpose

Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pRVL-5

Plasmid

#104583

Purpose

Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human IgG1 CH1/CH2/CH3 (IGHG1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pQmod2C-GG

Plasmid

#191347

Purpose

Clostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDS275

Plasmid

#34972

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A, I532A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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FLAG-MATR3

Plasmid

#32880

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pGL2-NOS2Promoter-Luciferase

Plasmid

#19296

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

Insert

NOS2 Promoter (Nos2 Mouse)

Use

Luciferase

Expression

Mammalian

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-RdLight1

Plasmid

#125706

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Insert

RdLight1

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pRVL-2

Plasmid

#104580

Purpose

Contains mouse IgG2c CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Mouse IgG2c CH1/CH2/CH3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_MMP-9-HA

Plasmid

#121172

Purpose

Expression of mouse MMP9

Depositor

M. Elizabeth Fini, Shinwu Jeong

Article

Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

Insert

MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV immediate/early

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVasa-Cre

Plasmid

#15885

Depositor

Diego Castrillon

Article

Gallardo et al Genesis. 2007 Jun . 45(6):413-7.

Insert

mouse vasa promoter (Ddx4 Mouse)

Tags

Cre

Expression

Mammalian

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pEK36

Plasmid

#210598

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter

Tags

mClover3

Expression

Bacterial and Yeast

Promoter

SpH2Bpro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pJAK184

Plasmid

#167281

Purpose

MazF-based mutagenesis vector for C. difficile

Depositor

Robert Fagan

Article

Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

Type

Empty backbone

Use

E. coli - c. difficile shuttle vector

Promoter

Pxyl

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pQmod2S-GG

Plasmid

#191346

Purpose

Clostridium expression vector (pBP1 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJAK080

Plasmid

#167279

Purpose

CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile

Depositor

Robert Fagan

Article

Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

Type

Empty backbone

Use

E. coli - c. difficile shuttle vector

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV Fcgamma RIIa IRES neo

Plasmid

#14948

Depositor

Axel Nohturfft

Article

Trivedi et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Fcgamma RIIa (FCGR2A Human)

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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