We narrowed to 23,976 results for: nes

1. pLPC-FLAG.Mm.EPC2

   Plasmid

   #184649

   Purpose

   Retroviral expression of mouse EPC2

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   Epc2 (Epc2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPC-FLAG.Mm.EPC1

   Plasmid

   #184648

   Purpose

   Retroviral expression of mouse EPC1

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   EPC1 (Epc1 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b+His SUMO PPM1H

   Plasmid

   #207333

   Purpose

   Bacterial expression of His SUMO PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBa.GFP-KIF16B tail 458-1317

   Plasmid

   #134624

   Purpose

   Mammalian expression of human KIF16B 458-1317

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF16B (KIF16B Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 457 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19_Ntag_BbsI-Mammalian

   Plasmid

   #186674

   Purpose

   N-terminal tag cassette with BbsI restriction sites for CRISPR donor plasmid.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   Mammalian N-terminal Tag
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKRG3-mU6-PUb-hSpCas9-pAc

   Plasmid

   #162163

   Purpose

   Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   T2A-pAc

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCKO

   Plasmid

   #73311

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAGM22082_cRed

   Plasmid

   #117225

   Purpose

   pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

   Insert

   Canthaxanthin Operon
   

   Use

   Tags

   34 amino acid N-terminal linker, Hexahistidine Ta…

   Expression

   Bacterial

   Mutation

   removed Type IIS restriction sites within the bac…

   Promoter

   T7 RNA Polymerase

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.LSL.tdTomato

   Plasmid

   #100048

   Purpose

   AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

    Plasmid

    #61665

    Purpose

    Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

    Depositor

    Kristen Verhey

    Article

    Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

    Insert

    KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB.GFP-KIF1A tail 395-1698

    Plasmid

    #134618

    Purpose

    Mammalian expression of mouse KIF1A 395-1698

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1A (Kif1a Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 394 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Cre-T2A-mRuby2

    Plasmid

    #102989

    Purpose

    Expresses Cre and mRuby2

    Depositor

    Karin Scharffetter-Kochanek

    Article

    Meyer et al PLoS Comput Biol. 2017 Dec 4;13(12):e100

    Inserts

    Cre (site-specific recombinase)
    mRuby2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBa.GFP-KIF1C tail 400-1103

    Plasmid

    #134621

    Purpose

    Mammalian expression of human KIF1C 400-1103

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1C (KIF1C Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 399 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

    Plasmid

    #134613

    Purpose

    Mammalian expression of rat KIF5C 378-955

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5C (Kif5c Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLPC-FLAG.Mm.KAT5

    Plasmid

    #184647

    Purpose

    Retroviral expression of mouse Kat5

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    Kat5 (Kat5 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKRG3-mU6-PUb-3xFLAG-hSpCas9

    Plasmid

    #162161

    Purpose

    Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. TOP2A sgRNA1

    Plasmid

    #138190

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TOP2A sgRNA2

    Plasmid

    #138191

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TOP2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

    Plasmid

    #162337

    Purpose

    Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6; mU6

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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