161,255 results

1. pAB2076 pAAV REPAIR.t1

   Plasmid

   #176323

   Purpose

   pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Inserts

   human codon optimized Cas13bt1 (catalytically inactivated)
   huADAR2dd(E488Q) (ADARB1 Human)
   Cas13bt1 crRNA + BpiI cloning site
   

   Use

   AAV and CRISPR

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   E488Q, only the deaminase domain (aa 276-702 are …

   Promoter

   EFS (short EF1alpha) and hU6

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pWPI-SLC7A11-HA

   Plasmid

   #201643

   Purpose

   Over-expression of HA-tagged human SLC7A11

   Depositor

   Alexis Jourdain, Vamsi Mootha

   Article

   Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti_MCP-p300_Hygro

   Plasmid

   #138459

   Purpose

   lenti vector encoding the MS2-p300 with a 2A Hygro resistance marker

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   p300 core
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-EGFP-Cdc42(wt)

   Plasmid

   #12599

   Depositor

   Klaus Hahn

   Article

   Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

   Insert

   EGFP-Cdc42 (WT) (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAG-TetON-3G

   Plasmid

   #96963

   Purpose

   Mammalian expression of Clontech rtTA driven by human beta-actin promoter

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV5-Smad7-HA

   Plasmid

   #11733

   Depositor

   Jeff Wrana

   Article

   Hayashi et al Cell. 1997 Jun 27. 89(7):1165-73.

   Insert

   SMAD7 (SMAD7 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pIVTR(T7A)-NGN2 (SA Substitutions)

   Plasmid

   #172308

   Purpose

   In vitro transcription of NGN2 (Ser phosphosites substituted by Ala)

   Depositor

   Patrik Verstreken

   Insert

   NGN2
   

   Use

   Other

   Tags

   Expression

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A an…

   Promoter

   T7 promoter A-initiating

   Available since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKL2351

   Plasmid

   #199721

   Purpose

   A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   NPTII
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET11b-SpyCatcher-HA2

    Plasmid

    #165991

    Purpose

    SpyCatcher-fused influenza HA ministem with removable HisTag

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyCatcher-HA2
    

    Use

    Tags

    Foldon, HisTag, and SpyCatcher

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge