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Showing: 3001 - 3030 of 5877 results

  1. pCDNA3-Flag-SPRTN-Y117C

    Plasmid
    #110216
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Y117C, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110216-plasmid-map-sequence-id-210941

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  2. pcDNA3-sACE2-T92Q-sfGFP

    Plasmid
    #145178
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145178-plasmid-map-sequence-id-292625

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  3. pcDNA3-sACE2-T92Q-Fc(IgA1)

    Plasmid
    #145162
    Purpose
    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Fc region of human IgA1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145162-plasmid-map-sequence-id-292598

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  7. pcDNA3-sACE2v2.4(732)-IgG1

    Plasmid
    #154106
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154106-plasmid-map-sequence-id-301643

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  8. pcDNA3-sACE2v2(732)-IgG1

    Plasmid
    #154105
    Purpose
    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Use
    Tags
    Fc region of human IgG1
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease and neck domains …
    Promoter
    CMV
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154105-plasmid-map-sequence-id-304581

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  9. pcDNA3-Xiap-Myc K322/328R

    Plasmid
    #11834
    Depositor
    Guy Salvesen
    Insert
    XIAP K322/328R (XIAP Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    K322/328R
    Promoter
    Available since
    May 12, 2006
    Availability
    Academic Institutions and Nonprofits only

  10. PME-1 S156A (inakt.mut) pcDNA-StrepIII

    Plasmid
    #119293
    Purpose
    Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center
    Depositor
    Jukka Westermarck
    Insert
    PPME1 (PPME1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    S156A
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    119293-plasmid-map-sequence-id-232194

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  11. pcDNA-GFP-EBA175-Flag(M1423R/P1424V/Y1425W/Y1426H)

    Plasmid
    #155313
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V/Y1425W/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V/Y1425W/Y1426H; insert codes for EBA…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155313-plasmid-map-sequence-id-310080

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  12. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/P1424Q/Y1426S)

    Plasmid
    #155009
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    E1418K/A1419R/P1424Q/Y1426S; insert codes for EBA…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155009-plasmid-map-sequence-id-309962

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  13. pcDNA-dCas9-p300 Core

    Plasmid
    #61357
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61357-plasmid-map-sequence-id-358013

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  15. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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Showing: 3001 - 3030 of 5877 results