We narrowed to 5,219 results for: Ado

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EF1a_KLF9_P2A_Hygro_Barcode

Plasmid

#120495

Purpose

Barcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF9 (KLF9 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-PGK-Puro

Plasmid

#110860

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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3019_pETcon-SARS-CoV-2-RBD_E484K

Plasmid

#184404

Purpose

yeast surface display of the SARS-CoV-2 Eta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

E484K

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_HOXA11_P2A_Hygro_Barcode

Plasmid

#120453

Purpose

Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA11 (HOXA11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL3_P2A_Hygro_Barcode

Plasmid

#120428

Purpose

Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL3 (ASCL3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF11_P2A_Hygro_Barcode

Plasmid

#120497

Purpose

Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF11 (KLF11 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1RA-P2A-Puro

Plasmid

#110853

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1RA

Use

Lentiviral

Tags

FLAG

Mutation

R661A, Q695A, Q926A, and NLS sequence at the N-te…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_HOXB6_P2A_Hygro_Barcode

Plasmid

#120454

Purpose

Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXB6 (HOXB6 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(259-443)

Plasmid

#89832

Purpose

Express GATA3 C1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C1 (AA 259-443)

Promoter

CMV

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-P2A-Puro

Plasmid

#110851

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181E

Plasmid

#105312

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181A

Plasmid

#105307

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF14_P2A_Hygro_Barcode

Plasmid

#120500

Purpose

Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF14 (KLF14 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit-NL

Plasmid

#113452

Purpose

Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine-NanoLuc

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

hUBbC

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

Plasmid

#184406

Purpose

yeast surface display of the SARS-CoV-2 Beta variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

K417N+E484K+N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-HF1-PGK-Puro

Plasmid

#110857

Purpose

Lentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-HF1

Use

Lentiviral

Tags

3X FLAG

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-VQRRA-PGK-Puro

Plasmid

#110858

Purpose

Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9-VQRRA

Use

Lentiviral

Tags

FLAG

Mutation

D1135V, R1335Q, T1337R and NLS sequence at the N-…

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_HOXA1_P2A_Hygro_Barcode

Plasmid

#120451

Purpose

Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

HOXA1 (HOXA1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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His 3C Kinase dead MKK6 1-334

Plasmid

#87882

Purpose

E. coli expression of MKK6 kinase dead

Depositor

Peter Mace

Article

Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

MKK6 (MAP2K6 Human)

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

K82A, kinase dead mutant

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-Fascin-Promoter (-402-0)

Plasmid

#89823

Purpose

Promoter luciferase assay

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Fascin1 (FSCN1 Human)

Use

Luciferase

Promoter

SV40

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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