We narrowed to 24,600 results for: Nes

1. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-3)

   Plasmid

   #159658

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

   Plasmid

   #159657

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2/V5 mmu-mir-208a

   Plasmid

   #26331

   Depositor

   David Bartel

   Article

   Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

   Insert

   mmu-mir-208a (Mir208a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

4. L309-myc-seipin-CT

   Plasmid

   #64063

   Purpose

   myc-tagged murine seipin (cytosolic C-terminus); lentiviral construct

   Depositor

   Weiping Han

   Article

   Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

   Insert

   bscl2 (Bscl2 Mouse)
   

   Use

   Lentiviral

   Tags

   myc epitope

   Expression

   Mammalian

   Mutation

   Contains Arg326 to Ser443

   Promoter

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

5. HOXB5 (human) pBluescript

   Plasmid

   #8531

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB5 (HOXB5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   makes protein in vitro transcription/translation

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pGEX6P1‐Afu rnhB

   Plasmid

   #108694

   Purpose

   For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Insert

   rnhB (AF_RS03165 Archaeoglobus fulgidus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG415-ADH1p-HA-rap1-K9R

   Plasmid

   #84531

   Purpose

   This plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   ADH1

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pAG415-GPDp-EGFP-rap1-K3NR

   Plasmid

   #84533

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(43,117,118)R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAG415-GPDp-EGFP-rap1-K3CR

   Plasmid

   #84534

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K(462,463,651)R

   Promoter

   GPD

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pAG415-GPDp-EGFP-rap1-K9R

    Plasmid

    #84535

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K(43,61,69,117,240,246,527,582,651)R

    Promoter

    GPD

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pDONR221-rap1-K462-463-651R

    Plasmid

    #84564

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K462-463-651R

    Promoter

    none

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-rap1-K43-117-118R

    Plasmid

    #84565

    Purpose

    This plasmid can be used as a donor for subcloning into Gateway destination vectors.

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Gateway donor plasmid

    Tags

    Expression

    Mutation

    K43-117-118R

    Promoter

    none

    Available Since

    Oct. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.2/V5 hsa-mir-220a

    Plasmid

    #26314

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-220a (MIR220A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pBa.GFP-KIF13AS1371D

    Plasmid

    #224417

    Purpose

    Mammalian expression of KIF 13a S1371D

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    KIF 13a S1371D (Kif13a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S1371D

    Promoter

    Chicken Beta actin

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBa.GFP-KIF13AS1371A

    Plasmid

    #224409

    Purpose

    Mammalian expression of KIF 13a S1371A

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    KIF 13a S1371A (Kif13a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    S1371A

    Promoter

    Chicken Beta actin

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBa.GFP-KIF13AWLDLE

    Plasmid

    #224410

    Purpose

    Mammalian expression of KIF 13a WLDLE

    Depositor

    Marvin Bentley

    Article

    Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

    Insert

    KIF 13a WLDLE (Kif13a Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    WLDLE

    Promoter

    Chicken Beta actin

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-C1-IFT57

    Plasmid

    #218728

    Purpose

    Expresses N-terminally EGFP-tagged IFT57 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Funabashi et al J Cell Biol. 2018 Aug 6;217(8):2867-2876

    Insert

    IFT57 (IFT57 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-UDP1.0-WPRE

    Plasmid

    #220849

    Purpose

    AAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)

    Depositor

    Yulong Li

    Article

    Umpierre et al Neuron. 2024 Apr 10:S0896-6273(24)00195-

    Insert

    UDP1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human Synapsin (hSyn)

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLPC-Flag-BirA-Linker-Myc-mRap1

    Plasmid

    #184644

    Purpose

    Retroviral expression of BirA-mRap1

    Depositor

    Agnel Sfeir

    Article

    Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

    Insert

    BirA-rap1
    

    Use

    Retroviral

    Tags

    BirA FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. puc19_N_tag

    Plasmid

    #186650

    Purpose

    Empty donor plasmid.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    N-terminal 3xFlag-3xHA Tag
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge