We narrowed to 13,053 results for: SHI

1. myc-hRAD18 dSAP

   Plasmid

   #68830

   Purpose

   Expresses c-myc-tagged human RAD18 deleting SAP domain

   Depositor

   Satoshi Tateishi

   Article

   Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

   Insert

   c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

2. myc-hRAD18 dC2

   Plasmid

   #68831

   Purpose

   Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain

   Depositor

   Satoshi Tateishi

   Article

   Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

   Insert

   c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

3. myc-hRAD18 d6BD

   Plasmid

   #68832

   Purpose

   Expresses c-myc-tagged human RAD18 deleting hRAD6 binding domain

   Depositor

   Satoshi Tateishi

   Article

   Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

   Insert

   c-myc tagged human RAD18 deleting hRAD6 binding domain (RAD18 Human)
   

   Use

   Tags

   c-myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pMALp2x-SCO5461(E164D)(43-204)

   Plasmid

   #42508

   Depositor

   Koji Okamoto

   Article

   Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

   Insert

   guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
   

   Use

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-42 (nucleotide 1-126); chan…

   Promoter

   tac

   Available since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pMRX-IP-EGFP-LOV2-OMP25

   Plasmid

   #233346

   Purpose

   Stable expression of EGFP-LOV2(N538E)-OMP25 in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

   Depositor

   Noboru Mizushima

   Article

   Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

   Inserts

   LOV2 domain of phototropin1
   Omp25 (Synj2bp Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N-terminus is deleted and N538E

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLINE1mORF1-ΔORF2-scFvFc

   Plasmid

   #206023

   Purpose

   The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

   Depositor

   Masamichi Kamihira

   Article

   Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

   Insert

   mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4-Xpress-GBF1(E794K)

   Plasmid

   #206418

   Purpose

   Mammalian expression of hGBF1(E794K)

   Depositor

   Kazuhisa Nakayama

   Article

   Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

   Insert

   GBF1 (GBF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E794K

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IRES-puro-3xFLAG

   Plasmid

   #204545

   Purpose

   Expresses 3xFLAG tag in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   3xFlag
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P-1-MVP

   Plasmid

   #204541

   Purpose

   Expresses GST-tagged MVP in E.coli

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   MVP (MVP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc

    Plasmid

    #186985

    Purpose

    AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guide

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-r1045-gggs-r1122, Gluc crRNA guide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    r1045-gggs-r1122 deletion

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-All-in-one-hCascade

    Plasmid

    #134919

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pBS-U6-crRNA-targeted. This plasmid coexpresses PuroR protein.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cascade factors (Cse1(Cas8), Cse2(Cas11), Cas5, Cas6, Cas7) with bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHes7-Achilles-Hes7

    Plasmid

    #153528

    Purpose

    Expresses fusion protein of Achilles/YFP and mouse Hes7 from mouse Hes7 promoter. The reporter shows oscillatory expression with 2-3 periodicity when expressed in mouse presomitic mesoderm.

    Depositor

    Ryoichiro Kageyama

    Article

    Yoshioka-Kobayashi et al Nature. 2020 Apr;580(7801):119-123. doi:

    Insert

    Hes7 (Hes7 Mouse)
    

    Use

    Tags

    Achilles

    Expression

    Mammalian

    Mutation

    Promoter

    mouse Hes7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pP{ELAV-GeneSwitch}

    Plasmid

    #83957

    Purpose

    Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

    Depositor

    Haig Keshishian

    Article

    Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

    Insert

    fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    elav

    Available since

    Dec. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRXIP-GFP-CERT(PHD)

    Plasmid

    #221746

    Purpose

    Stable expression of GFP-CERT(PHD)in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

    Insert

    CERT (CERT1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    amino acids 1–116

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid

    #233348

    Purpose

    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus is deleted (Cyb5a 95-134) and N538E

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

    Plasmid

    #172104

    Purpose

    Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. HR180-LGR5-iCT

    Plasmid

    #129094

    Purpose

    KI iCaspase9-tdTomato with a puromycin/mRuby selection cassette into human LGR5 locus

    Depositor

    Toshiro Sato

    Article

    Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

    Insert

    iCaspase9-T2A-tdTomato and LGR5 homology arms (LGR5 Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS0045

    Plasmid

    #131124

    Purpose

    Mammalian Target-ACE expression vector

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
    

    Use

    CRISPR

    Tags

    PmCDA1, TadA, and UGI

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

    Promoter

    CMV promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hPPP3CA-FLAG

    Plasmid

    #179134

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX209-neo-HA-Ub-T2A-Tet3G

    Plasmid

    #202436

    Purpose

    Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Ubiquitin
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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