We narrowed to 5,311 results for: cycs
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Plasmid#209250PurposeMammalian expression of ATP11B mutant E180QDepositorInsertATP11B (ATP11B Human)
UseTagsHAExpressionMammalianMutationE180QPromoterAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR
Plasmid#206213PurposePiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplificationDepositorInserttetracycline-responsive transactivator
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D41
Plasmid#185841PurposeTesting the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-PGK-SNAP-MDMUT-OCT4
Plasmid#115692PurposeConstitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for controlDepositorInsertSNAP-MDMUT-OCT4
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1pCNPRS
Plasmid#174719PurposetRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-5-pyridyl)alanine (pCNP), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A RNAi-resistant
Plasmid#163621Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112A and RNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationD570A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2-R232Q
Plasmid#124064PurposeControl protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATLDepositorInsertcytXlATL2-R232Q
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-dCys
Plasmid#110541PurposeBacterial protein of E.coli DHFR C85A/C152S mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationC85 mutated to A85, C152 mutated to S152PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBV163
Plasmid#108364PurposeUsed to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.DepositorInsertKnock-in Cg TDH3 promoter in Cg YAP1 gene
UseReplace c.glabrata yap1 promoter with c. glabrata…TagsExpressionMutationPromoterTDH3Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK360
Plasmid#71571PurposeProduces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)DepositorInsertacetyl-CoA:oxalate CoA-transferase
UseTagsExpressionBacterialMutationchanges threonine-2 to alaninePromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK385
Plasmid#71572PurposeProduces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)DepositorInsertsuccinyl-CoA:acetate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRevTre-mKLF5
Plasmid#41744DepositorInsertKlf5 (Klf5 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG593
Plasmid#40119DepositorInsertMex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationR274E, K318E, Exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailable sinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEG628
Plasmid#40120DepositorInsertMex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa1-244, Exon 2 recoded to be RNAi-resis…Promoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
tetO-MYOCD
Plasmid#170696Purposedoxycycline-inducible overexpression of MYOCDDepositorInsertMYOCD (MYOCD Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-SMAD6
Plasmid#170700Purposedoxycycline-inducible overexpression of SMAD6DepositorInsertSMAD6 (SMAD6 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-TBX20
Plasmid#170705Purposedoxycycline-inducible overexpression of TBX20DepositorInsertTBX20 (TBX20 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLCV2
Plasmid#87360PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.DepositorHas ServiceCloning Grade DNAInsertCas9-2A-eGFP
UseCRISPR and Lentiviral; Doxycycline inducible; egf…TagsCas9-T2A-eGFPExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tet-pLKO-neo
Plasmid#21916Purpose3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 22, 2009AvailabilityAcademic Institutions and Nonprofits only