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We narrowed to 25,261 results for: nes

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Showing: 2001 - 2020 of 25261 results

  6. pMJ923

    Plasmid
    #78313
    Purpose
    Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli
    Depositor
    Martin Jinek
    Article
    Burger et al Development. 2016 Apr 29. pii: dev.13480
    Insert
    SpCas9
    Tags
    HA-2xNLS-mCherry-NLS and His6-MBP-TEV
    Expression
    Bacterial
    Mutation
    human codon-optimized gene sequence
    Promoter
    T7
    Available Since
    June 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    78313-plasmid-map-sequence-id-148391

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  10. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid
    #192438
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.
    Depositor
    Jin Zhang
    Article
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Insert
    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    Tags
    Full-length LAMP1 and mCherry
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192438-plasmid-map-sequence-id-379134

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  12. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  14. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  19. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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  20. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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Showing: 2001 - 2020 of 25261 results