Addgene

We narrowed to 11,638 results for: pcdna

Showing: 3031 - 3060 of 11638 results

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Kohinoor-Tublin/pcDNA3

Plasmid

#67780

Purpose

Tublin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Use

Tags

b-Tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
pY008 (pcDNA3.1-hPbCpf1)

Plasmid

#69980

Purpose

Expresses humanized LPbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-flag-UTR

Plasmid

#183234

Purpose

Mammalian expression empty backbone

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Type

Empty backbone

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Rat NCX1.4 (pcDNA3.1+)

Plasmid

#75193

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-BIRC3-LU

Plasmid

#160700

Purpose

Expresses GFP-BIRC3 with the long 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7g1

Plasmid

#14624

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7g1 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
1-63 bcr/abl pcDNA3

Plasmid

#27479

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

(1-63) bcr/abl (ABL1, BCR Human)

Use

Tags

Expression

Mammalian

Mutation

Contains aa 1-63 of bcr and the complete abl chim…

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
Foxf2 pcDNA HisC

Plasmid

#122121

Purpose

Expression of Foxf2 in mammalian cells

Depositor

Paul Herring

Article

Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

Insert

Foxf2 (Foxf2 Mouse)

Use

Tags

6xHis and Express

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX6 (pcDNA3.1+)

Plasmid

#75215

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (Slc8b1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v3-sfGFP

Plasmid

#145174

Purpose

Mammalian expression plasmid for sACE2-sfGFP high affinity variant 3

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Linker GSGGSGSGG and superfolder GFP

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2cl-FLAG

Plasmid

#97077

Purpose

Expresses CARMA2cl/CARD14 isoform 2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al J Cell Physiol. 2011 Dec;226(12):3121-31

Insert

CARD14 isoform 2 (CARD14 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoNanobody IRES EGFP

Plasmid

#136621

Purpose

Plasmid encoding outer mitochondrial localization of the Enhancer nanobody for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-EnhancerNb (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/v5-DEST hGlut9

Plasmid

#18088

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

Glut9 (SLC2A9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only
pY123 (pcDNA3.1-huCMaCpf1)

Plasmid

#92299

Purpose

Expression of humanized CMaCpf1

Depositor

Feng Zhang

Insert

huCMaCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pY125 (pcDNA3.1-huBfCpf1)

Plasmid

#92301

Purpose

Expression of humanized BfCpf1

Depositor

Feng Zhang

Insert

huBfCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY119 (pcDNA3.1-huLb5Cpf1)

Plasmid

#92295

Purpose

Expression of humanized Lb5Cpf1

Depositor

Feng Zhang

Insert

huLb5Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pY115 (pcDNA3.1-huMlCpf1)

Plasmid

#92275

Purpose

Expression of humanized MlCpf1

Depositor

Feng Zhang

Insert

huMlCpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRTTO-NHASt-codoptUS2

Plasmid

#135102

Purpose

Codon-optimized HSV-1 US2 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 US2 codon optimized

Use

Tags

HA-Strep-Strep

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Use

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-MSL3L1

Plasmid

#29761

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

MSL3L1 (MSL3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12C (Trim12c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v6-Fc(IgG1)

Plasmid

#145169

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 6

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R2ACR_142-EYFP

Plasmid

#103964

Purpose

Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R2ACR_142

Use

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12A

Plasmid

#87061

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12A (Trim12a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp7-HF

Plasmid

#157690

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-V5-DGCR8-mDRBD1&2

Plasmid

#51385

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

DRBD1 & DRBD2 mutant (A568K, A569K, A676K, S6…

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
Y771F EphA2 pcDNA3

Plasmid

#102735

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y771F (TAC > TTC)

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Y593E EphA2 pcDNA3

Plasmid

#102731

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Y593E (TAC > GAG)

Promoter

CMV

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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