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YIplac211-VPS8-iYFPx6

Plasmid

#105272

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Tags

iYFPx6

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-GaCYP722C(pYL777)

Plasmid

#178290

Purpose

Expresses GaCYP722C from G. arboreum in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GaCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

GaCYP722C

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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p416PGK1-OsCYP711A2(pYL770)

Plasmid

#178301

Purpose

Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A2

Expression

Yeast

Promoter

PGK1

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pXP214

Plasmid

#26829

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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NAT-TET-OFF -LEU- 4xFLAG

Plasmid

#182527

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

4XFLAG

Expression

Yeast

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS8-iGFPx6

Plasmid

#105271

Purpose

Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps8 portion (VPS8 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-HTB-hphMX4

Plasmid

#26876

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-HIS

Plasmid

#160016

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1

Plasmid

#112646

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1

Expression

Yeast

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAGT572_Nemo

Plasmid

#153487

Purpose

Level 1 episomal expression plasmid for S. cerevisiae

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pPKC1 (JCE575)

Plasmid

#89773

Purpose

yeast expression of PKC1

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC1 (PKC1 Budding Yeast)

Expression

Yeast

Promoter

yeast PKC

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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Pyb1301(JBEI-11629)

Plasmid

#87959

Purpose

jStack yeast assembly into plant vectors, Level 2 pYB Vector, Hygro selection

Depositor

Dominique Loque

Article

Shih et al Nat Commun. 2016 Oct 26;7:13215. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pILGFP1F5

Plasmid

#165064

Purpose

Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-RGS18HIS-hphMX4

Plasmid

#26883

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

18xHis tag

Use

Pcr tagging yeast

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-HPH

Plasmid

#160207

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-LEU

Plasmid

#160176

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-LEU

Plasmid

#160029

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-HIS

Plasmid

#160021

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pXP618

Plasmid

#26848

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pKT-SCFP3A-HIS

Plasmid

#64686

Purpose

pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

SCFP3A

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pXP412

Plasmid

#26840

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pXP314

Plasmid

#26835

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pUC57-8xHis

Plasmid

#101699

Purpose

8xHis in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

AATG-[8xHis]-AGCC

Use

Yeast expression

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pXP122

Plasmid

#26827

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Expression

Yeast

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pHpgRNA50

Plasmid

#160128

Purpose

To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1

Depositor

Yongjin Zhou

Article

Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

Insert

PTEF1-HH-gRNA-LSC2- HDV-TAMO

Expression

Yeast

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBM4607

Plasmid

#18795

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Sir4 (SIR4 Budding Yeast)

Tags

myc

Expression

Yeast

Mutation

aa 951-1200

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pXP518

Plasmid

#26846

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pR184

Plasmid

#47952

Purpose

rtTA positive feedback loop with three tet operators in the GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Expression

Yeast

Promoter

Gal1

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS16-iGFPx6

Plasmid

#105275

Purpose

Plasmid for pop-in/pop-out replacement of Vps16 with Vps16-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps16 portion (VPS16 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge