Addgene

We narrowed to 9,706 results for: yeast

Showing: 3031 - 3060 of 9706 results

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pAG414GPD-GmCYP722C(pYL887)

Plasmid

#178298

Purpose

Expresses GmCYP722C from G. max in Saccharomyces cerevisiae. Centromeric TRP, PGPD-GmCYP722C-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

GmCYP722C

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-FvCYP722C2(pYL878)

Plasmid

#178299

Purpose

Expresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

FvCYP722C2

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-OsCYP711A3(pYL761)

Plasmid

#178300

Purpose

Expresses OsCYP711A3 from O. sativa in Saccharomyces cerevisiae. Centromeric TRP, PGPD-OsCYP711A3-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A3

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pR184

Plasmid

#47952

Purpose

rtTA positive feedback loop with three tet operators in the GAL1 promoter

Depositor

Attila Becskei

Article

Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

Insert

rtTA

Use

Tet inducible expression

Tags

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pBM4607

Plasmid

#18795

Depositor

Mark Johnston

Article

Wang et al Nat Protoc. 2008;3(10):1569-77. doi: 10.

Insert

Sir4 (SIR4 Budding Yeast)

Use

Tags

myc

Expression

Yeast

Mutation

aa 951-1200

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pMM2_4

Plasmid

#183057

Purpose

HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mRuby2
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mRuby2 and mTurquoise2

Expression

Yeast

Mutation

Promoter

HAA1 and YGP1

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_10

Plasmid

#183059

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsYGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Mutation

Promoter

HAA1 and Synthetic promoter

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMM2_11

Plasmid

#183060

Purpose

Control for biosensor in pMM2_9: HAA1-mTurquoise2 under HAA1p and mCherry under HREsENO1cp with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mCherry
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Mutation

Promoter

HAA1 and Synthetic promoter

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pI4-MTU-DO-HIS

Plasmid

#160026

Purpose

Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-HPH

Plasmid

#160019

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Hygromycin selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pI2-MTU-DO-LEU

Plasmid

#160017

Purpose

Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Leucine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Tags

Expression

Yeast

Mutation

Promoter

n/a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJAID8Z

Plasmid

#165053

Purpose

Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>SKP1>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID6A

Plasmid

#165046

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>TAgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-msGFP-VPS21

Plasmid

#105265

Purpose

Plasmid for pop-in/pop-out replacement of Vps21 with msGFP-Vps21.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps21 (VPS21 Budding Yeast)

Use

Tags

msGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416 Hsh155 WT

Plasmid

#87219

Purpose

Plasmid used to maintain viability after deletion of genomic copy of HSH155.

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-TRP1-PGAL1

Plasmid

#41606

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

TRP1 reverse orientation

Expression

Mutation

Promoter

Available since

May 15, 2013

Availability

Academic Institutions and Nonprofits only

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pMM0624

Plasmid

#128999

Purpose

pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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EC2_1_dCas9_sgRNA

Plasmid

#163710

Purpose

Yeast low copy plasmid with dCas9 and sgRNA expression cassette

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Use

Tags

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinADE GFP

Plasmid

#32120

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Promoter

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pILGFP5V1

Plasmid

#165058

Purpose

Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1- T-URA3

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGADT7 Hsh155 WT

Plasmid

#87288

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 WT

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pPS1601

Plasmid

#8921

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

PAB1 (PAB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pRS406-GFP-SNC1(L96V)

Plasmid

#105278

Purpose

Integrating construct for expressing GFP-Snc1(L96V) from the TPI1 promoter.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snc1 (SNC1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Leucine 96 changed to Valine, intron removed

Promoter

TPI1

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGBKT7-PRP9

Plasmid

#87593

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp9

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP9 (PRP9 Budding Yeast)

Use

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBKT7-PRP43

Plasmid

#87596

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp43

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP43 (PRP43 Budding Yeast)

Use

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPAP001

Plasmid

#153488

Purpose

Level 1 episomal expression plasmid for P. pastoris

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p415 GFP-Glo3

Plasmid

#112658

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

sfGFP-Glo3

Use

Tags

sfGFP

Expression

Yeast

Mutation

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-BIO-hphMX4

Plasmid

#26880

Depositor

Peter Kaiser

Article

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

BIO-tag

Use

Pcr-tagging yeast

Tags

Expression

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
413-Met25 Kar2 TorsinA K108A GFP

Plasmid

#32125

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Use

Tags

GFP, HDEL, and Kar2

Expression

Yeast

Mutation

K108A

Promoter

Available since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only
pGBKT7-PRP5

Plasmid

#87592

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp5

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Use

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge