We narrowed to 4,010 results for: SPL;

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pGL3_4xUAS_CP-candidate_luc+

Plasmid

#125154

Purpose

STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

4 x upstream activating sequence (UAS)

Use

Luciferase

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pET:ACAT:GFP

Plasmid

#60740

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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3158 pSFFV-neo Bad cDNA

Plasmid

#8753

Depositor

Stanley Korsmeyer

Article

Yang et al Cell 1995 Jan 27;80(2):285-91.

Insert

BAD (Bad Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-deltaNLS1-EGFP

Plasmid

#22150

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 297-304

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEIIIA

Plasmid

#14059

Depositor

Richard Hynes

Article

Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

Insert

Rat Fibronectin EIIIA (Fn1 Rat)

Expression

Mammalian

Mutation

EcoRI linkers added to TaqI to HinfI fragment

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pM215A

Plasmid

#173217

Purpose

Expresses ScFV-GCN4-sFGFP10M2-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

ScFV-GCN4-sfGFP10M2-GB1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pM214

Plasmid

#173216

Purpose

Expresses MCP-mCherry-GFP11-GB1

Depositor

Hajin Kim

Article

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

MCP-mCherry-GFP11-GB1

Use

CRISPR and Lentiviral

Tags

HA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pET:CCCT:GFP

Plasmid

#60738

Purpose

A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CCCT' between -11 and -8.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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p3x-Flag Gli3 30R3-1

Plasmid

#120304

Purpose

Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 30C

Depositor

David Liu

Article

Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

Insert

30R3-1, Gli3

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI135

Plasmid

#126073

Purpose

Vector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI166

Plasmid

#126075

Purpose

Vector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI128

Plasmid

#126209

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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3156 pBS Bad cDNA

Plasmid

#8752

Depositor

Stanley Korsmeyer

Article

Yang et al Cell 1995 Jan 27;80(2):285-91.

Insert

BAD (Bad Mouse)

Expression

Bacterial

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pcFN-C (chick fibronectin)

Plasmid

#14063

Depositor

Richard Hynes

Article

Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

Insert

Chicken Fibronectin (FN1 Chicken)

Expression

Mammalian

Mutation

DraI - EcoRI fragment inserted in HincII-EcoRI si…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pNMHCII-C2-GFP

Plasmid

#46043

Depositor

Robert Adelstein

Article

Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

Insert

nonmuscle myosin heavy chain II-C, isoform C2 (Myh14 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV immediate early

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only
pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PRMT9 G260E mutant

Plasmid

#67610

Purpose

bacterial expression of GST-PRMT9 G260E Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

G260E double E loop mutant

Promoter

tac

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 D258G mutant

Plasmid

#67609

Purpose

bacterial expression of GST-PRMT9 D258G Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

D258G double E loop mutant

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
PLL7.0 tgRFP LOV

Plasmid

#114463

Purpose

Mammalian expression of tgRFP labelled LOV2

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

LOV2

Tags

tgRFP

Expression

Mammalian

Mutation

G401A(G528A), N411E(N538E) and V289I (V416I)

Promoter

CMV

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoPL

Plasmid

#114467

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoPL

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoPL

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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