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Addgene

We narrowed to 3,857 results for: SPL;

Showing: 2021 - 2040 of 3857 results
  1. NODAL-MYC-DYK-N72A-N199A

    Plasmid
    #115252
    Purpose
    For mammalian expression of the human NODAL open reading frame (with two mutated N-glycosylation sites) with a C-terminal MYC-DYK tag
    Depositor
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    N72A, N199A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. NODAL-MYC-DYK-C312A

    Plasmid
    #115253
    Purpose
    For mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tag
    Depositor
    Insert
    NODAL (NODAL Human)
    Use
    Tags
    MYC-DYK
    Expression
    Mammalian
    Mutation
    C312A
    Promoter
    CMV
    Available since
    Sept. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. Rp51a_10bp 5'SS_No BP or pBP_pUC18

    Plasmid
    #100989
    Purpose
    Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

    Plasmid
    #100990
    Purpose
    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation
    Depositor
    Insert
    RP51A (RPS17A Budding Yeast)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…
    Promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pMRBad-C-DiB-RM

    Plasmid
    #168478
    Purpose
    C-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Insert
    C-fragment of DiB-RM
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pET11a-N-DiB-RM

    Plasmid
    #168476
    Purpose
    N-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split protein
    Depositor
    Insert
    N-fragment of DiB-RM
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pET11a-Z-N-wtBlc

    Plasmid
    #168258
    Purpose
    N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain wtBlc‐split-Zip protein
    Depositor
    Insert
    N-fragment of wtBlc + Leucine Zipper
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pMRBad-C-wtBlc

    Plasmid
    #168257
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168259: pET11a-N-wtBlc or Plasmid 168472: pET11a-N-DiB2 to obtain wtBlc‐split or DiB2‐split protein, correspondingly
    Depositor
    Insert
    C-fragment of wtBlc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pMRBad-Z-C-wtBlc

    Plasmid
    #168256
    Purpose
    C-fragment. This plasmid can be co-transformed with either Plasmid 168258: pET11a-Z-N-wtBlc or Plasmid 168473: pET11a-Z-N-DiB2 to obtain wtBlc‐split-Zip or DiB2‐split-Zip protein, correspondingly
    Depositor
    Insert
    Leucine Zipper + C-fragment of wtBlc
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    araBAD
    Available since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. IBMc120

    Plasmid
    #161950
    Purpose
    Contains elements for building reporter plasmid for IBM. Built upon pSB4A3. ECF20 reporter plasmid
    Depositor
    Insert
    pSB4A3-PECF20-B30-mScarlet-I-B15
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PECF20
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 2021 - 2040 of 3857 results