We narrowed to 2,367 results for: clu

1. pEGFPC2-gephyrin C4a

   Plasmid

   #199611

   Purpose

   Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C4a casette insertion

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV sgRNA-Notch1 #2 GFP

   Plasmid

   #106951

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV sgRNA-Notch1 #3 GFP

   Plasmid

   #106952

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pX459-HypaCas9-H2BC11_sgRNA

   Plasmid

   #183883

   Purpose

   pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX459-HypaCas9-mR2-H2BC11_sgRNA

   Plasmid

   #183881

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFlare9A-CD45i4e5i5mutCBS

   Plasmid

   #131835

   Purpose

   A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   PTPRC (PTPRC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   partial intron 4 full exon 5 and partial intron 5…

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA0057

   Plasmid

   #131784

   Purpose

   Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

   Depositor

   Frank Albert

   Article

   Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

   Insert

   20mer GFP cassette guide (gGFP) and 5' sgRNA
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHBS1039 TDP43 RRM-GFP 2xΦ

   Plasmid

   #107806

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined to two clusters in LLPS…

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHBS1038 TDP43 RRM-GFP 4xΦ

   Plasmid

   #107805

   Purpose

   To test the effect of sequence on TDP43 droplet properties

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined to four clusters in LLP…

   Promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHBS1037 TDP43 RRM-GFP 6xΦ

    Plasmid

    #107804

    Purpose

    To test the effect of sequence on TDP43 droplet properties

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    All hydrophobics combined to six clusters in LLPS…

    Promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRevLCav2FeS

    Plasmid

    #60808

    Purpose

    to disrupt iron sulfur cluster in yeast Rev3

    Depositor

    Tahir Tahirov, Youri Pavlov

    Article

    Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

    Insert

    REV3 (REV3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    contains 647 C-terminal amino acids and 335 bp of…

    Promoter

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pTH443-SUP45-G180A

    Plasmid

    #29386

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G180A mutant gene including genomic sequence 1432…

    Promoter

    Available since

    June 22, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pTH398-SUP45-G54D

    Plasmid

    #29376

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G54D mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pTH372-SUP45-K60A

    Plasmid

    #29379

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K60A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pTH394-SUP45-L34S

    Plasmid

    #29373

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L34S mutant gene (corresponding to the sup45-36ts…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pTH355-SUP45-T55A

    Plasmid

    #29377

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T55A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pTH373-SUP45-S61A

    Plasmid

    #29380

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S61A mutant gene including genomic sequence 1432 …

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pTH401-SUP45-R65C

    Plasmid

    #29381

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R65C mutant gene (corresponding to the sup45-3 al…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pTH627-SUP45-V107D

    Plasmid

    #29382

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V107D mutant gene including genomic sequence 1432…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pTH441-SUP45-K175T

    Plasmid

    #29384

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K175T mutant gene including genomic sequence 1432…

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only