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We narrowed to 24,925 results for: crispr

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Showing: 2021 - 2040 of 24925 results

  1. pPgiCas13b-gRNA-NT

    Plasmid
    #184835
    Purpose
    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.
    Depositor
    Chase Beisel
    Publication
    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2
    Insert
    Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Nov. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184835-plasmid-map-sequence-id-377028

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  2. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

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  4. ABE9

    Plasmid
    #194208
    Purpose
    Mammalian expression of ABE9
    Depositor
    Dali Li
    Publication
    Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-8e (N108Q/L145T)-nSpCas9
    Use
    CRISPR
    Tags
    6*His
    Expression
    Mammalian
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    194208-plasmid-map-sequence-id-473532

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  7. MO91-dCas9-AID(P182X)

    Plasmid
    #83260
    Purpose
    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.
    Depositor
    Xing Chang
    Publication
    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.
    Insert
    dCas9-AID P182X
    Use
    Retroviral
    Tags
    3xFLAG-NLS and FLAG
    Expression
    Mammalian
    Mutation
    D10A and H840A in Cas9; P182X in AID
    Promoter
    MSCV
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles

  8. pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant

    Plasmid
    #101210
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S355C/S867C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S355C, S867C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101210-plasmid-map-sequence-id-198133

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  9. pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant

    Plasmid
    #101212
    Purpose
    Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/S701C/S960C/N497A/R661A/Q695A/Q926A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, S701C, S960C, N497A, R661A, Q695A an…
    Promoter
    T7
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    101212-plasmid-map-sequence-id-197040

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  12. U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti

    Plasmid
    #135955
    Purpose
    Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b
    Depositor
    David Liu
    Publication
    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586
    Insert
    Prime editor v2 (PE2) N-terminal
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    hSynapsin
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    135955-plasmid-map-sequence-id-269512

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  14. pX330-HA-dSpCas9

    Plasmid
    #92112
    Purpose
    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9
    Use
    CRISPR
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92112-plasmid-map-sequence-id-197503

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  15. pMOD_A1710

    Plasmid
    #91039
    Purpose
    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSP
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-P2A-TaCas9_H840A nickase
    Use
    CRISPR
    Mutation
    H840A
    Promoter
    ZmUbi
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91039-plasmid-map-sequence-id-181793

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Showing: 2021 - 2040 of 24925 results