We narrowed to 23,976 results for: nes

1. TRRAP sgRNA2

   Plasmid

   #138183

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRRAP

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TRRAP (TRRAP Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SIN-PGK-Cas9-V5-WPRE

   Plasmid

   #87876

   Purpose

   To produce lentiviral vector for Cas9 editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Human codon-optimized

   Promoter

   mouse PGK

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HOXB4 (human) FLAG MSCV

   Plasmid

   #8528

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Missing N-terminal 14 amino acids; improved Ribos…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pBa.GFP-KIF17 tail 400-1038

   Plasmid

   #134617

   Purpose

   Mammalian expression of mouse KIF1A 400-1038

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF17 (Kif17 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 399 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

   Plasmid

   #162336

   Purpose

   Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6; mU6

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-RoninDHSA-FLAG-IRES-Neo

   Plasmid

   #28021

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Insert

   Ronin (THAP11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   DHSA mutation (Y246A) of the previously defined H…

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. HOXB4 (human) HA-TAT-tag pET

   Plasmid

   #8526

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial

   Mutation

   replace T7 and HIS tags of pET with an HA tag;

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. SIN-CMV-Cas9-V5-WPRE

   Plasmid

   #87904

   Purpose

   To produce lentiviral vector for gene editing

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   Cas9-V5
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   human codon-optimized

   Promoter

   CMV

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-ER-E2F2-dRb

   Plasmid

   #62805

   Purpose

   expresses an inducible E2F2 mutant that lacks the Rb binding domain

   Depositor

   Gerardo Ferbeyre

   Article

   Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

   Insert

   E2F2 (E2F2 Human)
   

   Use

   Retroviral

   Tags

   HA-Er

   Expression

   Mammalian

   Mutation

   deletion of amino acids 302-437, including the Rb…

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. p1.2-GS

    Plasmid

    #162770

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SIN-PGK-Cas9-WPRE

    Plasmid

    #87886

    Purpose

    To produce Lentiviral vector for gene editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    hCas9
    

    Use

    Lentiviral

    Tags

    nls

    Expression

    Mutation

    Human codon-optimized

    Promoter

    mouse PGK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCKO_PSMB2_sgRNA_1

    Plasmid

    #74184

    Purpose

    lentiviral vector expressing sgRNA targeting PSMB2

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMB2 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSC2

    Plasmid

    #91180

    Purpose

    T-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting NCR genes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMP71-tCD19-llo-mc38tmg

    Plasmid

    #174603

    Purpose

    expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes ADGPK, DPAGT and Reps1

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    extracell transmem domains moCD19 wCterm fusion LLO190 and minigenes of 3neoantigens MC38tumor in genes ADGPK DPAGT Reps1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. L309-myc-seipin-ΔCT

    Plasmid

    #64062

    Purpose

    myc-tagged murine seipin with deletion of cytosolic C-terminus); lentiviral construct

    Depositor

    Weiping Han

    Article

    Yang et al Biochem J. 2013 May 15;452(1):37-44. doi

    Insert

    bscl2 (Bscl2 Mouse)
    

    Use

    Lentiviral

    Tags

    myc epitope

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal Asp338–Ser443

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

16. SIN-CMV-Ca9-WPRE

    Plasmid

    #87874

    Purpose

    Transfer plasmid for the production of lentiviral vectors

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    nls

    Expression

    Mutation

    Human codon-optimized

    Promoter

    CMV

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLCKO_PSMB2_sgRNA_5

    Plasmid

    #74185

    Purpose

    lentiviral vector expressing sgRNA targeting PSMB2

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    PSMB2 sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-hs-3xHA-C12AC15A-LIN-41

    Plasmid

    #52725

    Purpose

    retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    changed cysteines 12 and 15 to alanines

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG415-GPDp-EGFP-rap1-K651R

    Plasmid

    #84536

    Purpose

    This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

    Depositor

    Jorrit Enserink

    Article

    Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

    Insert

    RAP1 (RAP1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    K651R

    Promoter

    GPD

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTC362

    Plasmid

    #91216

    Purpose

    protoplast vector for targeted deletion of 6 genes in tomato

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting 6 tomato genes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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