159,557 results

1. pShuttle-PR.Cre

   Plasmid

   #192933

   Purpose

   pShuttle.Cre encoding sgRNAs targeting Trp53 and Rb1 genes

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Insert

   Trp53, Rb1 (Trp53, Rb1 Mouse)
   

   Use

   Gateway-compatible vector

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 9, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDB.His.MBP.3C CARD8 CARD WT

   Plasmid

   #164022

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX68

   Plasmid

   #46857

   Purpose

   OP Hupki-puro2A-A138V plasmid to indroduce mutant p53 to PLF ESC or MEFs by IMCE

   Depositor

   Monica Hollstein

   Article

   Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

   Insert

   attB-Puro-2A and Hupki p53 A138V (TP53 Human, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   p53 A138V

   Promoter

   Available since

   Aug. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiFUCCI(CA)5

   Plasmid

   #223176

   Purpose

   Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

   Depositor

   Neville Sanjana

   Article

   Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

   Insert

   FUCCI(CA)5
   

   Use

   Lentiviral

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-PE7-P2A-hMLH1dn

   Plasmid

   #222997

   Purpose

   Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

   Depositor

   Brittany Adamson

   Article

   Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

   Insert

   PE7-P2A-hMLH1dn
   

   Use

   Tags

   SV40 bpNLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Deletion of residues 754-756 in MLH1 as described…

   Promoter

   CMV

   Available since

   Aug. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIRV-NFAT-eGFP

   Plasmid

   #118031

   Purpose

   Fluorescent reporter for NFAT activation

   Depositor

   Peter Steinberger

   Article

   Jutz et al J Immunol Methods. 2016 Jan 15. pii: S00

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   minimal promoter with NFAT binding site

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-PVX

   Plasmid

   #213040

   Purpose

   PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Cauliflower mosaic virus 35S

   Available since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB1.HMBP.PrS.MTF2

   Plasmid

   #125169

   Purpose

   Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   MTF2 (MTF2 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti6-H2B-mCherry

    Plasmid

    #89766

    Purpose

    Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    Histone H2B (H2BC11 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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