2,226 results

1. Myc-gamma1/gamma2SIL

   Plasmid

   #121432

   Purpose

   GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)
   

   Use

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PCDHGC5 sh1 shRNA

   Plasmid

   #122227

   Purpose

   Knock Down Protocadherin gamma C5. It targets Protocadherin gamma C5 mRNA (nucleotides 851–871 in the protein coding region).

   Depositor

   Angel de Blas

   Article

   Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

   Insert

   PCDHGC5 shRNA (Pcdhgc5 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PCDHGC5 sh1 3m shRNA

   Plasmid

   #122228

   Purpose

   Negative control shRNA (with 3-point mutations), for plasmid 122227: targeting Protocadherin gamma C5 mRNA.

   Depositor

   Angel de Blas

   Article

   Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

   Insert

   PCDHGC5 shRNA (Pcdhgc5 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOTTC1079 - pAAV TH gRNA A EF1a EGFP

   Plasmid

   #113159

   Purpose

   An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   gRNA for rat TH
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

   Plasmid

   #113155

   Purpose

   An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat TH
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

   Plasmid

   #113157

   Purpose

   An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat MANF
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 and hU6

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. rat brain inositol 1,4,5-trisphosphate 3-kinase A

   Plasmid

   #122155

   Purpose

   rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase A; clone CP16 (full length)

   Depositor

   Christophe Erneux

   Article

   Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

   Insert

   inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET24a-VHH-tev-2xTS

   Plasmid

   #117750

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to two tyrosine sulfation (TS) motifs. VHH-2xTS also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, TS, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and Tyrosine sulfation (TS) se…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SEPT11 sh2 3m shRNA

   Plasmid

   #122024

   Purpose

   Negative control shRNA for SEPT11 sh2 shRNA (plasmid 122023).

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 shRNA (Sept11 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Geph UTR3m shRNA

    Plasmid

    #121071

    Purpose

    Negative control shRNA for plasmid 121070: shRNA targeting the 3'-untranslated region (UTR) of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gamma2 UTR shRNA (shRNA4)

    Plasmid

    #120796

    Purpose

    shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. gamma2 CR3m shRNA

    Plasmid

    #120795

    Purpose

    Negative control shRNA for plasmid 120794: shRNA targeting the coding region of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. gamma2 UTR3m shRNA

    Plasmid

    #120797

    Purpose

    Negative control shRNA for plasmid 120796: shRNA targeting the 3'-untranslated region (UTR) of the gamma2 mRNA

    Depositor

    Angel de Blas

    Article

    Li et al J Neurochem. 2005 Nov;95(3):756-70. doi:

    Insert

    GABRG2 shRNA (Gabrg2 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Myc-gamma1 (in pCAGGS)

    Plasmid

    #118039

    Purpose

    GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG1 (Gabrg1 Rat)
    

    Use

    Tags

    cMyc epitope EQKLISEEDL inserted between fourth a…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX4T3-Stx3-L77A/L80A

    Plasmid

    #100273

    Purpose

    Expresses GST-Stx3-L77A/L80A in bacteria.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

    Insert

    Stx3-1-265-L77A/L80A (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain; residues 4-264 present, …

    Promoter

    Tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX4T3-Stx3-E78A/E83A

    Plasmid

    #100272

    Purpose

    Expresses GST-Stx3-E78A/E83A in bacteria.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

    Insert

    Stx3-1-265-E78A/E83A (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain; residues 4-264 present, …

    Promoter

    Tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX4T3-Stx3-LE165/166AA

    Plasmid

    #100271

    Purpose

    Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

    Depositor

    Thomas Weimbs

    Article

    Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

    Insert

    Stx3-1-265-LE165/166AA (Stx3 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    No transmembrane domain; residues 4-264 present, …

    Promoter

    tac

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pR2Q191y

    Plasmid

    #101029

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Use

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pR2Q207y

    Plasmid

    #101024

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    Glutamate receptor subunit (Gria2 Rat)
    

    Use

    Tags

    fluorescent protein

    Expression

    Mammalian

    Mutation

    tagged with fluorescent protein

    Promoter

    cMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFlg240

    Plasmid

    #101018

    Purpose

    fluorescent, functional, glutamate receptor subunit

    Depositor

    Thomas Hughes

    Article

    Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

    Insert

    GluR 1 (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fluorescent protein inserted into subunit

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge