2,294 results

1. pOC2 Gria1-GFP KI

   Plasmid

   #183430

   Purpose

   CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX552-rScn2a-3xV5-Syn-miRFP670

   Plasmid

   #182566

   Purpose

   For rat Nav1.2 knockin with 3xV5 at the extracellular loop

   Depositor

   James Zhe Liu

   Article

   Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

   Inserts

   rat Scn2a gRNA and 3xV5 donor DNA
   miRFP670
   

   Use

   AAV and CRISPR

   Promoter

   Syn and U6

   Available Since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRB131

   Plasmid

   #180174

   Purpose

   pAAV construct GluN1A714L (shRNA resistant)

   Depositor

   Richard Bergeron

   Article

   Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

   Insert

   GluN1 (Grin1 Rat)
   

   Use

   AAV

   Tags

   Phluorin

   Expression

   Mammalian

   Mutation

   A714L

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLIC-His-GBAi W211A

   Plasmid

   #171756

   Purpose

   For the expression of His-GBAi W211A in bacteria

   Depositor

   Mikel Garcia-Marcos

   Article

   Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

   Insert

   GBAi
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Nov. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGEX-4T Doc2beta C2AB(125) H158C

   Plasmid

   #134691

   Purpose

   Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Double C2 protein beta (Doc2b Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   H158C, C145A, C217A, C249A, C290A, C338A, C387A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T Doc2beta C2AB(125) F222C

   Plasmid

   #134692

   Purpose

   Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Double C2 protein beta (Doc2b Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F222C, C145A, C217A, C249A, C290A, C338A, C387A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T Doc2beta C2AB(125) A298C

   Plasmid

   #134693

   Purpose

   Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Double C2 protein beta (Doc2b Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   A298C, C145A, C217A, C249A, C290A, C338A, C387A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T Syt1 C2AB(140) M173C

   Plasmid

   #134696

   Purpose

   Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Synaptotagmin 1 (Syt1 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   M173C, C277A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T Syt1 C2AB(140) F234C

   Plasmid

   #134697

   Purpose

   Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

   Insert

   Synaptotagmin 1 (Syt1 Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F234C, C277A

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-4T Syt1 C2AB(140) V304C

    Plasmid

    #134698

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    V304C, C277A

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T Syt1 C2AB(140) I367C

    Plasmid

    #134699

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    I367C, C277A

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVT-EGFP-ratCENP-C

    Plasmid

    #174445

    Purpose

    in vitro transcription template for rat CENP-C (N-ter EGFP tagged)

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Centromere protein C
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1535 pscAAV mU6 shRNA(scram) CMV-IE Nuc-EYFP

    Plasmid

    #135563

    Purpose

    An AAV vector expressing scrambled shRNA and a nuclear EYFP reporter

    Depositor

    Christopher Richie

    Article

    Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

    Inserts

    shRNA (scrambled)
    Nuc-EYFP
    

    Use

    AAV and RNAi

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    CMV-IE and mU6

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIC-His-GBAi

    Plasmid

    #171755

    Purpose

    For the expression of His-GBAi in bacteria

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

    Plasmid

    #167478

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(I539E)-ShadowG
    

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

    Plasmid

    #167479

    Purpose

    Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(C450A)-ShadowG
    

    Tags

    ShadowG and mEGFP(A206K)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM10 with rat Δ192Annexin A11

    Plasmid

    #164498

    Purpose

    For bacterial expression of rat His-tagged Δ192Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ192Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM10 with rat Δ188Annexin A11

    Plasmid

    #164497

    Purpose

    For bacterial expression of rat His-tagged Δ188Annexin A11

    Depositor

    Anni Vedeler

    Article

    Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

    Insert

    rat Δ188Annexin A11 (Anxa11 Rat)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    N-terminal truncation

    Promoter

    T7

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ad-HisFlag-USP19ER

    Plasmid

    #155244

    Purpose

    for generating adenoviruses expressing the ER isoform of rat USP19

    Depositor

    Simon Wing

    Article

    Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

    Insert

    USP19 (ER isoform) (Usp19 Rat)
    

    Use

    Adenoviral

    Tags

    6XHis-3XFlag

    Mutation

    H118R- please see dep. comments

    Promoter

    CMV

    Available Since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIS-rPAM3

    Plasmid

    #145803

    Purpose

    expresses rat PAM3 in mammalian cells

    Depositor

    Richard Mains, Betty Eipper

    Article

    Milgram et al J Cell Biol. 1994 Jan;124(1-2):33-41. do

    Insert

    rat PAM3
    

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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