We narrowed to 10,045 results for: Coli
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Plasmid#65006PurposeE. coli vector for expression of S. pyogenes dCas9, tracrRNA, and nontargeting CRISPR array with BsaI site for inserting user-defined spacer-repeat bricksDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive native promotersAvailable SinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only
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pET41a iGluu
Plasmid#119834PurposeProduction of recombinant iGluSnFR S72T ultrafast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluu
TagsGSTExpressionBacterialMutationS72TPromoterT7Available SinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pM9-BetaGal-8C
Plasmid#172489PurposePlasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequenceDepositorInsertM9-BetaGal-8C
Tags6xHis and M9 nuclear localization sequence (from …ExpressionBacterialMutationBetaGal has two cysteine mutations (C77A, C1022S)…PromoterT7Available SinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b/bPanK
Plasmid#73619PurposeProduces Escherichia coli pantothenate kinase, with N-terminal His6 tag (H6PanK)DepositorInsertpantothenate kinase (coaA Escherichia coli)
TagsHis6ExpressionBacterialMutationrelative to MG1655 sequence, changes glutamate-5 …PromoterT7Available SinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2_mScarletI_mVenusNB-MrkHR113A
Plasmid#182292Purposeexpresses c-di-GMP mutant biosensor in E. coliDepositorInsertmVenusNB-MrkHR113A
TagsmVenusNB internal fusion to MrkH with a R113A poi…ExpressionBacterialMutationchanged arginine 113 to alanine, MrkH numbering …PromoterproCAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PatoL-OG241
Plasmid#72841PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). OG241 backbone.DepositorInsertPatoL
ExpressionBacterialPromoterPatoLAvailable SinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR
Plasmid#119828PurposeProduction of recombinant iGluSnFR, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR
TagsGSTExpressionBacterialPromoterT7Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-His-TEV-OTC_Ub
Plasmid#172134PurposeScaffold design for studying ubiquitin interacting proteins by cryo-EM.DepositorInsertE. coli ornithine transcarbamylase (OTC), fused to human ubiquitin
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t-gfp
Plasmid#48130PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
TagsGSTExpressionBacterialMutationE25DPromoterT7Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30b GluBP600n
Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
TagsHisExpressionBacterialPromoterT7Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlul
Plasmid#119832PurposeProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlul
TagsGSTExpressionBacterialMutationT92APromoterT7Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1-gfp
Plasmid#48133PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b-GB
Plasmid#129689Purposeexpress E. coli GreBDepositorAvailable SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t-gfp
Plasmid#48131PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t-gfp
Plasmid#48132PurposeShuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoterDepositorInsertgfp
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only