We narrowed to 13,580 results for: ckb

1. pLX304-IRES-Td

   Plasmid

   #176296

   Purpose

   pLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sites

   Depositor

   William Kaelin

   Article

   Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. XLone-Puro Cas13d-eGFP U6 BbsI

   Plasmid

   #155184

   Purpose

   PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Unspecified; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV/eGFP-U6-sgRNA

   Plasmid

   #170544

   Purpose

   A lentiviral backbone for homology directed insertion of eGFP. Containing only eGFP, flanked by restriction sites for insertion of homology arms and a sgRNA casstte to clone in sgRNA for HDR

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PDG458 V3

   Plasmid

   #226959

   Purpose

   CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. PX458 V3

   Plasmid

   #226957

   Purpose

   CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dual-CRISPR-Cas9-U6-H1 (pBP48)

   Plasmid

   #218932

   Purpose

   The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   2A-Puro and 3XHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_Ef1a:PE2-P2A-GFP

   Plasmid

   #184445

   Purpose

   Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Ef1A

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTA-Mob 2.0 Tp

   Plasmid

   #188619

   Purpose

   Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Conjugation helper plasmid

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLCKO

   Plasmid

   #73311

   Purpose

   Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB46 (CK2beta)

    Plasmid

    #27085

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. BLADE

    Plasmid

    #134912

    Purpose

    Empty backbone for cloning sgRNA sequence to be used in nanoblades system

    Depositor

    Emiliano Ricci, Philippe Mangeot, Théophile Ohlmann

    Article

    Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZ699

    Plasmid

    #208117

    Purpose

    Payload yeast assembly vector with TK-mActb on backbone

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    Synthetic Biology; Copy number inducible

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Human PGK1 for thymidine kinase

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZW12 (CK2beta)

    Plasmid

    #27088

    Depositor

    David Litchfield

    Article

    Turowec et al Methods Enzymol. 2010;484:471-93.

    Insert

    CK2beta (CSNK2B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-TRC.mKO2

    Plasmid

    #85208

    Purpose

    Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a Wb-iPGM

    Plasmid

    #180246

    Purpose

    Bacterial expression plasmid for production of recombinant Wuchereria bancrofti iPGM His10

    Depositor

    James Inglese

    Article

    van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

    Insert

    Wuchereria bancrofti iPGM-10His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-VTKS1

    Plasmid

    #170852

    Purpose

    AAV backbone for transgene expression in neurons under the control of the hSyn1 promoter.

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB PUb-integrase

    Plasmid

    #183966

    Purpose

    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    PhiC31 integrase
    

    Use

    Piggybac insect transgenesis destination vector

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Use

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SaCas9v2-Puro

    Plasmid

    #178802

    Purpose

    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available Since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCHKOv3-mCherry

    Plasmid

    #209035

    Purpose

    Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mCherry and puromycin selection marker

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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