We narrowed to 14,886 results for: pET

1. p3521 pET ubcH6

   Plasmid

   #8653

   Depositor

   Peter Howley

   Article

   Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

   Insert

   UbcH6 (UBE2E1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

2. RanBP2-RB3-4-pET23a

   Plasmid

   #85769

   Purpose

   bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

   Depositor

   Frauke Melchior

   Article

   Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

   Insert

   RanBP2 fragment (RANBP2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   fragment (amino acids 2304-3062)

   Promoter

   T7

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-SUMO-SpyTag003

   Plasmid

   #216282

   Purpose

   Expresses SUMO with a C-terminal SpyTag003 in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SUMO-SpyTag003
   

   Use

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b-rrt-His8

   Plasmid

   #194088

   Purpose

   Expresses Ec86 reverse transcriptase in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 rrt
   

   Use

   Tags

   His8

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-GFP-mHP1alpha

   Plasmid

   #181912

   Purpose

   Bacterial protein expression vector for GFP-tagged HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-ymYPET-CaURA3

   Plasmid

   #118460

   Purpose

   Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymYPET
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   YPET A206K, F208S, E232L, N235D

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1-L713F

   Plasmid

   #174794

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1-C125G

   Plasmid

   #174792

   Purpose

   Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-C125G (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C125G, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM33_TNKS_TANK-peptide-bind

   Plasmid

   #178489

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   TNKS_TANK-peptide-bind (TNKS Human, Synthetic)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7/LacO

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28b-ETV6-ETS

    Plasmid

    #111180

    Purpose

    Minimal ETS domain of human ETV6 (TEL)

    Depositor

    Gregory Poon

    Article

    Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

    Insert

    ETV6 ETS domain (ETV6 Human, Mouse, Bovine)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBabe-CyPet-Rac1

    Plasmid

    #24534

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Retroviral

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pET21-pelB-LaG-11

    Plasmid

    #172762

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-11
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET29b-Ava3858-3855

    Plasmid

    #45746

    Depositor

    Emily Balskus, Christopher T Walsh

    Article

    Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

    Insert

    Ava3858-3855
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pET51b(+)_SNAP-p24

    Plasmid

    #130718

    Purpose

    Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-p24
    

    Use

    Tags

    Hisx10 and StrepTag

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET3C-ARL16(CO)-His6

    Plasmid

    #216485

    Purpose

    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

    Depositor

    Richard Kahn

    Insert

    ADP-ribosylation factor-like 16 (ARL16 Human)
    

    Use

    Tags

    includes GSGSGSHHHHHH (His6) tag at C-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM6-RgTALsyn-HpaBC

    Plasmid

    #100949

    Purpose

    Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Inserts

    RgTALsyn
    HpaB
    HpaC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-PLS-KP6_rpoA-U

    Plasmid

    #190967

    Purpose

    Test the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coli

    Depositor

    Bernard Gutmann

    Article

    Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

    Insert

    PLS-KP6
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaM-1

    Plasmid

    #172767

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-1, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-1
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21(+)-His6-Ubc13

    Plasmid

    #212715

    Purpose

    Bacterial expression of His6-Ubc13

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    Ubc13
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge