We narrowed to 1,286 results for: MS2;
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Plasmid#153546Purposethis is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucineDepositorInserthuman TCR ζ-Chain (CD247 Human)
ExpressionMammalianMutationwith 6 Y in 3 ITAMs mutated to LPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP
Plasmid#154139PurposeExpresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.DepositorInsertdCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
Tags3x FLAG and tagBFPExpressionMammalianPromoterCMVAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a(R887E)-DNMT3l
Plasmid#154141PurposeExpresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianMutationR887EPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a-DNMT3l
Plasmid#154140PurposeExpresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
ExpressionBacterialAvailable SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z
Plasmid#153543Purposeperform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publicationDepositorAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-delCry2
Plasmid#153545Purposethis is the non-clustering version of LIC-Z tagged with mcherryDepositorInserthuman TCR ζ-Chain (CD247 Human)
TagsmCherryExpressionMammalianMutationwith Cry2 sequence deleted from LIC-ZPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo
Plasmid#160458PurposeHomology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTRDepositorUseInsertion of a fluorescent reporter through homol…Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-YFP
Plasmid#153544Purposeperform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publicationDepositorInserthuman TCR ζ-Chain (CD247 Human)
TagsVenus YFPExpressionMammalianMutationwith mCherry in LIC-Z replaced by YFPPromoterCMVAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57-Cx46-IRES-GCaMP6s
Plasmid#188238Purpose3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6sDepositorInsertGJA3 (GJA3 Human)
UseLentiviralTagsGCaMp6sExpressionMammalianPromoterTight TRE promoterAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only