We narrowed to 16,205 results for: She

1. pY129 (pcDNA3.1-U6-huMb3Cpf1)

   Plasmid

   #92305

   Purpose

   Expression of humanized Mb3Cpf1 and crRNA

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTre-tight-SV40-luciferase (496)

   Plasmid

   #65489

   Purpose

   Inducible expression of coding/noncoding genes

   Depositor

   Roderic Guigo, Rory Johnson

   Insert

   SV40-luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight Tre promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CYP2C19

   Plasmid

   #69606

   Purpose

   For protein expression of CYP2C19

   Depositor

   Joyce Goldstein

   Insert

   CYP2C19 (CYP2C19 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deletion of AA1-8 and mutation I331V

   Promoter

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

   Plasmid

   #133572

   Purpose

   CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTXB3-Flag PLK1

   Plasmid

   #68271

   Purpose

   bacterial expression of Plk1

   Depositor

   Stefano Ferrari

   Insert

   PLK1 (PLK1 Human)
   

   Use

   Tags

   Flag and Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pUPRT::Floxed DHFR-TS*

   Plasmid

   #100606

   Purpose

   TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.

   Depositor

   David Sibley

   Insert

   TgUPRT::Floxed TgDHFR-TS*
   

   Use

   Toxoplasma expression

   Tags

   Expression

   Mutation

   Pyrimethamine resistance

   Promoter

   pTgDHFR-TS

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PB CAG-L-bpA-3xSV40pA-L-td-sfGFP

   Plasmid

   #133577

   Purpose

   CAG tdGFP cre/lox reporter piggyBac transposon (bpA 3x SV40pA stop)

   Depositor

   Mario Capecchi

   Insert

   Tandem dimer superfolder GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK645

   Plasmid

   #71700

   Purpose

   Produces E. coli thioredoxin (EcTrxA)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HT-ELK1

   Plasmid

   #33258

   Depositor

   Frederick Stanley

   Insert

   ELK1 (ELK1 Human)
   

   Use

   Tags

   Halo Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

10. PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

    Plasmid

    #133573

    Purpose

    CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTRIPZ (M)-YFP-Eed

    Plasmid

    #82512

    Purpose

    Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.

    Depositor

    Xiaojun Ren

    Insert

    embryonic ectoderm development
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    insert contains AA39-441 of EeD

    Promoter

    cmv

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET3a-DJ1

    Plasmid

    #51489

    Purpose

    Inducible expression of human DJ1 in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. PB CAG-L-bpA-2xSV40pA-L-td-sfGFP

    Plasmid

    #133576

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 2x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Clover2-C1

    Plasmid

    #54711

    Purpose

    Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    Clover2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSAS)

    Plasmid

    #137864

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAGSAS N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG416GAL-3x-uC-ccdB-6stop

    Plasmid

    #203514

    Purpose

    Gateway-compatible destination vector for yeast expression with GAL1 promoter (3x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG416GAL-2x-uA-ccdB-6stop

    Plasmid

    #203517

    Purpose

    Gateway-compatible destination vector for yeast expression with GAL1 promoter (2x GAL4 binding sites)/upstream ORF/6stop mutation/URA3 marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKK1 -pFlp-18::mito4x-GCaMP6f

    Plasmid

    #194969

    Purpose

    Fluorescent reporter of mitochondrial matrix Ca2+.

    Depositor

    Frederic Hoerndli

    Insert

    4 repeats of Cox8 presequence plus GCaMP6f
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Flp-18

    Available since

    June 21, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pBEST-pTar-UTR1-TetR-T500

    Plasmid

    #45780

    Depositor

    Richard Murray, Vincent Noireaux

    Insert

    Tetracycline repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTar

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-(neg27)GFP-GGS9-Cre-6xHis

    Plasmid

    #89253

    Purpose

    Expresses GFP with -27 theoretical charge fused to Cre in E.Coli

    Depositor

    David Liu

    Insert

    (neg27)GFP-GGS9-Cre-6xHis
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMZ376

    Plasmid

    #74213

    Purpose

    Combined sgRNA/Cas9 vector with KanMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. sCFP in pBSDONR P4r-P2

    Plasmid

    #121530

    Purpose

    For making C-terminal sCFP fusions using multisite Gateway cloning

    Depositor

    Roger Innes

    Insert

    sCFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE2-RSV-jGCaMP7s-W

    Plasmid

    #126690

    Purpose

    Mammalian calcium sensor protein.

    Depositor

    Darrell Kotton

    Insert

    jGCaMP7s
    

    Use

    Lentiviral

    Tags

    6xHIS, T7, Xpress

    Expression

    Mutation

    A52V, A317L

    Promoter

    RSV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUltra-Smurf-luc-RTTA3

    Plasmid

    #59438

    Purpose

    silence doxycyclin inducible pUltra-dox (also expresses luciferase)

    Depositor

    Kitai Kim, Yildirim Dogan

    Inserts

    luciferase
    Reverse TET-Transactivator 3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    loxP5171

    Promoter

    Available since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV Tor1a-V5

    Plasmid

    #175135

    Purpose

    Lentiviral expression of Tor1a-Myc

    Depositor

    Larry Gerace

    Insert

    Tor1a (Tor1a Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE2-RSV-jGCaMP7f-W

    Plasmid

    #126691

    Purpose

    Mammalian calcium sensor protein.

    Depositor

    Darrell Kotton

    Insert

    jGCaMP7f
    

    Use

    Lentiviral

    Tags

    6xHIS, T7, Xpress

    Expression

    Mutation

    H78K, A317L

    Promoter

    RSV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMC6-TEFkFLP(LYS2)

    Plasmid

    #49458

    Purpose

    overespression of Flp step-arrest mutant in yeast, LYS2 selection

    Depositor

    Marc Gartenberg

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    TEF1

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBluescript mouse ptc

    Plasmid

    #58699

    Purpose

    full length mouse ptc cDNA

    Depositor

    Matthew Scott

    Insert

    ptc (Ptch1 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pJK266

    Plasmid

    #73326

    Purpose

    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    violacein biosynthesis protein VioD
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pIC81

    Plasmid

    #63065

    Purpose

    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    PMFI, IMAGE clone 6148250 (PMF1 Human)
    

    Use

    Tags

    EYFP and TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid

    #133575

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PTEN-VC

    Plasmid

    #89332

    Purpose

    expresses hPTEN fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    PTEN1 (PTEN Human)
    

    Use

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. B0030m_BC

    Plasmid

    #74394

    Purpose

    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]

    Depositor

    Douglas Densmore

    Insert

    RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSBtet-Bla/GFP

    Plasmid

    #198221

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP

    Depositor

    Eric Davis

    Insert

    EGFP
    

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. H2B CFP

    Plasmid

    #198059

    Purpose

    mCerulean fused to human H2B protein

    Depositor

    Amro Hamdoun

    Insert

    H2B
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mCerulean

    Expression

    Mutation

    Promoter

    sp6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pJK690

    Plasmid

    #72380

    Purpose

    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)

    Depositor

    T. J. Kappock

    Insert

    glutaredoxin-4
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJK409

    Plasmid

    #72423

    Purpose

    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK405

    Plasmid

    #72418

    Purpose

    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJK403

    Plasmid

    #72416

    Purpose

    Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MAC045-C12

    Plasmid

    #58753

    Purpose

    bacterial expression of PDB 4NXJ

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4NXJ
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available since

    Aug. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits