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pBEL2521

Plasmid

#195643

Purpose

Complementation of MlaC(A34R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

MlaC(A34R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2512

Plasmid

#195626

Purpose

Complementation of MlaA(delta238-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(delta238-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2513

Plasmid

#195627

Purpose

Complementation of MlaA(delta227-251)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(delta227-251)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL2639

Plasmid

#195628

Purpose

Complementation of MlaA(I241N)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaA

Expression

Bacterial

Mutation

MlaA(I241N)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1160

Plasmid

#196025

Purpose

Expression/purification of 6xHis-TEV-MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1161

Plasmid

#196026

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta141-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta141-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBEL1195

Plasmid

#196027

Purpose

Complementation of MlaF-MlaE-MlaD-MlaC-MlaB

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaFEDCB

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-NcPAN2_1-461_Y

Plasmid

#148104

Purpose

Bacterial Expression of NcPAN2_1-461

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

NcPAN2_1-461

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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GB2566

Plasmid

#193104

Purpose

Minimal promoter of SlDFR gene containing 62bp upstream the transcription start site and the 5'UTR region.

Depositor

Diego Orzaez

Article

Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

Insert

GB_SynP minSlDFR

Use

Synthetic Biology

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- HTA1prom-TIR1-FLAG

Plasmid

#189725

Purpose

Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

HTA1

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pPpT4- TEF2prom-TIR1-FLAG

Plasmid

#189726

Purpose

Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

Depositor

Anita Emmerstorfer-Augustin

Article

Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

Insert

OsTIR1

Tags

FLAG

Expression

Yeast

Promoter

TEF2

Available Since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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InterTag-1xL

Plasmid

#186911

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-(GGGGS)1-PDGFR

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTopo RE2 Donor pLox hPGK-Puro pA pLox

Plasmid

#171049

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-Cas9-EGFP-L2

Plasmid

#141045

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9-2A-EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-FokI-dCas9-L2

Plasmid

#141042

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-FokI-dCas9

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Cas9n-EGFP-L2

Plasmid

#141044

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9n-2A-EGFP

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-Cas9n-L2

Plasmid

#141041

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9n

Expression

Mammalian

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Cas9-EGFP-L2

Plasmid

#141043

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9-2A-EGFP

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-SpCas9-HF4-2A-EGFP-L2

Plasmid

#141049

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF4-2A-EGFP

Expression

Mammalian

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-Cas9-L2

Plasmid

#141040

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge