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pICH85281

Plasmid

#50272

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Promoter + 5'UTR, mas, (A. tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGM5343

Plasmid

#50297

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus) + signal peptide, RAmy3A (O. sativa) + HIS tag

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pFM115

Plasmid

#40161

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa850-1286)

Promoter

tac

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFP118

Plasmid

#21671

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
800 pME-Bozozok

Plasmid

#49008

Purpose

full length coding sequence for zebrafish bozozok gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

dharma (dharma )

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRabin8_5

Plasmid

#26714

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rabin8 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rabin8 alpha isoform RNAi

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pWZ414-F14

Plasmid

#23063

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Expression

Yeast

Mutation

Histone H3 deleted amino acids 3-29

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_2

Plasmid

#26711

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Rab25.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I Y248&249F

Plasmid

#22189

Depositor

Ananda Roy

Article

Novina et al J Biol Chem. 1998 Dec 11. 273(50):33443-

Insert

TFII-I (GTF2I Human)

Tags

GST

Expression

Mammalian

Mutation

Changed Tyrosines 248 and 249 to Phenylalanines.

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pJB15x02

Plasmid

#247094

Purpose

In vivo expression of wild-type Enterocin RJ-11

Depositor

Erika DeBenedictis

Article

Pulschen et al Nat Commun. 2026 Mar 24. doi: 10.1038/s4

Insert

Enterocin RJ-11 (WT)

Expression

Bacterial

Mutation

N/A

Available Since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_mTagBFP2

Plasmid

#250898

Purpose

mTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mTagBFP2

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux*_R1_EGFP

Plasmid

#250901

Purpose

EGFP expressed under PLux* promoter; part of BNeu AS14; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Mutation

PLux* = Point mutation in PLux

Promoter

PLux*

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLux*_R1_mKO2

Plasmid

#250902

Purpose

mKO2 expressed under PLux* promoter; component of BNeu AS14*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

mKO2

Use

Synthetic Biology

Mutation

PLux* = Point mutation in PLux

Promoter

PLux*

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_J23102_R1_LuxR

Plasmid

#250887

Purpose

LuxR regulator protein expressed under BBa_J23102 promoter; part of BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

LuxR

Use

Synthetic Biology

Promoter

BBa_J23102

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PJ23102R1LuxR_PCTR1E2Crimson

Plasmid

#250892

Purpose

LuxR expressed under BBa_J23102; E2-Crimson under PCT synthetic promoter; component of BNeuAS6; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

E2-Crimson
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLLacO1_R1_EGFP

Plasmid

#250897

Purpose

EGFP expressed under PL LacO1 promoter; part of BNeu AS11; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL LacO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PLTetO1_R1_EGFP

Plasmid

#250899

Purpose

EGFP expressed under PL TetO1 promoter; part of BNeu AS12; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

EGFP

Use

Synthetic Biology

Promoter

PL TetO1

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pM2_PCTR1EGFP_PJ23102R1LuxR

Plasmid

#250885

Purpose

EGFP expressed under PCT synthetic promoter and LuxR expressed under BBa_J23102 promoter; component of BNeuAS3; colE1 origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Inserts

EGFP
LuxR

Use

Synthetic Biology

Promoter

BBa_J23102 and PCT

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PHLT_R1_E2Crimson

Plasmid

#250886

Purpose

E2-Crimson expressed under PHLT synthetic promoter; component of BNeuAS4; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PHLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pK1_PLT_R1_E2Crimson

Plasmid

#250882

Purpose

E2-Crimson expression under PLT synthetic promoter; part of BNeu AS1*; p15A origin of replication

Depositor

Sangram Bagh

Article

Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534

Insert

E2-Crimson

Use

Synthetic Biology

Promoter

PLT

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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