We narrowed to 62,913 results for: cloning

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7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Publication

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Expression

Mammalian

Promoter

7 repeats of Gli biding site

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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AAVS1-idCas9-vpr

Plasmid

#89985

Purpose

inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

Depositor

Jie Na

Publication

Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

Insert

dCas9-VPR

Expression

Mammalian

Promoter

TRE-tight

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Publication

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SS9_RNA

Plasmid

#71656

Purpose

SS9-targeting gRNA for co-transformation with pSS9

Depositor

Ryan Gill

Publication

Bassalo et al ACS Synth Biol. 2016 Apr 22.

Insert

SS9 gRNA

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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CHMP2A_GFP_N_term

Plasmid

#31805

Depositor

Daniel Gerlich

Publication

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

CHMP2A (CHMP2A Human)

Expression

Mammalian

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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phND2-N106

Plasmid

#31780

Depositor

Jerry Crabtree

Publication

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

NeuroD2 (NEUROD2 Human)

Use

Lentiviral

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDR-GW AT1.03YEMK

Plasmid

#28004

Depositor

Wolf Frommer

Publication

Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

Insert

AT1.03YEMK

Tags

mVenus and mseCFP

Expression

Yeast

Mutation

M60Y, G131E, K132M, 133K

Available since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pDONR223-NTRK3

Plasmid

#23901

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK3 (NTRK3 Human)

Use

Gateway Cloning

Mutation

F441L and L653P relative to NM_001012338.3

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-RET

Plasmid

#23906

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RET (RET Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pENTREGFP2

Plasmid

#22450

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP (eGFP )

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Flag-HA-EIF3S3

Plasmid

#22545

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

EIF3S3(1-348aa) (EIF3H Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pQCXP CMV/TO DEST (w360-1)

Plasmid

#17386

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Retroviral

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAd NeuroD-I-nGFP

Plasmid

#19414

Depositor

Douglas Melton

Publication

Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Adenoviral and Gateway Cloning

Tags

GFP and IRES

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pDEST17-UbE2D3

Plasmid

#15784

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)

Tags

His

Expression

Bacterial

Mutation

see Depositor Comments below

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX_DSB_Spectrum-V3

Plasmid

#192476

Purpose

Lentiviral DSB Spectrum V3 Reporter System

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, mCherry, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Promoter

hPGK

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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HR_G3BP1-V5-APEX2-GFP

Plasmid

#105284

Purpose

homologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locus

Depositor

Eugene Yeo

Publication

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

G3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 Human, soybean)

Use

Human homologous recombination donor

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Publication

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Publication

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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