We narrowed to 10,315 results for: plasmids 101

1. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

   Plasmid

   #112669

   Purpose

   Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   tdT
   

   Use

   AAV; Donor plasmid for recombinase-mediated casse…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Kv1.1 K+ channel scFv [K20/78]

   Plasmid

   #190486

   Purpose

   Mammalian Expression of Kv1.1 K+ channel scFV. Derived from hybridoma K20/78.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Kv1.1 K+ channel (Rattus norvegicus) recombinant scFV (Kcna1 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCHC042 (∆phaCAB)

   Plasmid

   #226208

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pZac2.1 GfaABC1D rM3D mCherry SV40

   Plasmid

   #92285

   Purpose

   AAV DREADD expression

   Depositor

   Baljit Khakh

   Article

   Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

   Insert

   rM3D
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS2340_Tier3(SB)-iRFP_Puro

   Plasmid

   #169638

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven iRFP670 and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCHC039 (∆phcA)

   Plasmid

   #226207

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for PhcA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pTS2337_Tier3(SB)-TagBFP2_Puro

   Plasmid

   #169635

   Purpose

   Tier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven mTagBFP2 and PuroR expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PRPBSA

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221-rap1-K9R

   Plasmid

   #84527

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   K(43,61,69,117,240,246,527,582,651)R

   Promoter

   none

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pSBL_DK193

   Plasmid

   #156424

   Purpose

   Insect cell expression of 3a(Q57H)-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (Q57H)
   

   Use

   Tags

   sfGFP-His

   Expression

   Insect

   Mutation

   Q57H

   Promoter

   Polyhedrin

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BirA_BVES untagged

    Plasmid

    #196581

    Purpose

    expresses BirA

    Depositor

    Cheryl Arrowsmith

    Article

    Alteen et al Structure. 2023 Sep 7;31(9):1121-1131.e6

    Insert

    BirA_Ecoli
    

    Use

    Baculovirus expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 9, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pProvector_AAV2_586_PLGLAR

    Plasmid

    #129024

    Purpose

    Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position 586.

    Depositor

    Junghae Suh

    Article

    Tong et al J Control Release. 2019 Jun 25. pii: S01

    Insert

    Rep2/Cap2 with PLGLAR lock at 586
    

    Use

    AAV

    Tags

    Expression

    Mutation

    inserted peptide motif (PLGLARGDDDDGPLGLAR) at po…

    Promoter

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SARS-CoV-2 Nucleocapsid

    Plasmid

    #242452

    Purpose

    Expresses the SARS-CoV-2 Nucleocapsid protein

    Depositor

    Benjamin Schuster

    Article

    Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

    Insert

    Nucleocapsid protein
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SCML2_3

    Plasmid

    #220878

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Article

    Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

    Insert

    SCML2 (SCML2 Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA 3.1-nsp7

    Plasmid

    #201026

    Purpose

    Expresses SARS-CoV-2 nsp7 in mammalian.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTS2343_Tier3(SB)-YPet_Blast

    Plasmid

    #169641

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-YPet-p2A-BlastR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet and BlastR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS2344_Tier3(SB)-mRuby2_Blast

    Plasmid

    #169642

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby marker and BlastR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-BlastR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven mRuby2 and BlastR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS2339_Tier3(SB)-Ruby_Puro

    Plasmid

    #169637

    Purpose

    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven mRuby2 and PuroR expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRPBSA

    Available Since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.2 mir-1-1 reporter mir44-4

    Plasmid

    #46707

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    mir44-4 (mir-44 Nematode, Synthetic)
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    see sequence and publication

    Promoter

    CMV

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.2 mir-1-1 reporter cel-mir-43-44 context

    Plasmid

    #46654

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    cel-mir-43-44 context
    

    Use

    RNAi

    Tags

    V5 and hsa-mir-1-1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Hy_pMT dati MCS circfirefly_1677

    Plasmid

    #110118

    Purpose

    Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge