We narrowed to 5,413 results for: PEP;

1. pEN-W-A-ccdB-G-X

   Plasmid

   #218891

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-W-ccdB-Z

   Plasmid

   #218890

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCTCON2-Aga2p-Flag-CapN-TEVcs-HA

   Plasmid

   #213530

   Purpose

   Expresses CapN-caged TEVcs on yeast surface

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   Aga2p-Flag-CapN-TEVcs-HA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Gal1/10

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. plx208-CMV-SspB-EGFP-VP16

   Plasmid

   #213532

   Purpose

   Expresses one of the split transcription factor component in mammalian cells

   Depositor

   Wenjing Wang

   Article

   Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

   Insert

   SspB-EGFP-VP16
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA TfR1-TinyERRE

   Plasmid

   #208324

   Purpose

   C-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TfR1-TinyERRE
   

   Use

   Tags

   TinyERRE

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA TfR1-PunyEEER

   Plasmid

   #208326

   Purpose

   C-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TfR1-PunyEEER
   

   Use

   Tags

   PunyEEER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1BR9-BFP

   Plasmid

   #202054

   Purpose

   E. coli expression vector for N-ter GFP11 and C-ter R9 tagged tagBFP.

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   BFP
   

   Use

   Tags

   6xHis, GFP11, and R9

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1BR9-BFPSTOP

   Plasmid

   #202055

   Purpose

   E. coli expression vector for N-ter GFP11 R9 tagged BFP

   Depositor

   Markita Landry

   Article

   Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

   Insert

   BFP
   

   Use

   Tags

   6xHis and GFP11

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB511-TP-UnaG-FLAG

   Plasmid

   #203479

   Purpose

   For Agrobacterium transformation. A plastid transit peptide fused UnaG-FLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   Plastid transit peptide fused UnaG-FLAG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB511-TP-tagRFP

    Plasmid

    #203482

    Purpose

    For Agrobacterium transformation. tagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    TagRFP flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB602-TP-mCherry-UnaG

    Plasmid

    #203483

    Purpose

    For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    Plastid transit peptide fused mCherry-UnaG
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. cytoDCIP

    Plasmid

    #193861

    Purpose

    Encodes mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    mCherry-GFP1-10
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGADT7-AP-1 σ1B

    Plasmid

    #198335

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGADT7-AP-1 σ1A

    Plasmid

    #198334

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 σ1A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-1 σ1A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Expresses a LELQMNRRY nonapeptide after the last …

    Promoter

    ADH1

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVRC-N-Venus-V150A-apCC-Di

    Plasmid

    #190706

    Purpose

    Encodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.

    Depositor

    Nigel Savery

    Article

    Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

    Insert

    N-Venus-V150A-apCC-Di
    

    Use

    Tags

    Expression

    Mutation

    V150A

    Promoter

    araBAD

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET23PI-Sce ESARC D218A

    Plasmid

    #195608

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T928C, T929C, A942C

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET23PI-Sce ESARC

    Plasmid

    #195607

    Purpose

    Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

    Insert

    PI-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

    Promoter

    T7

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. VAMP8

    Plasmid

    #186689

    Purpose

    Expresses VAMP8 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    VAMP8
    

    Use

    Tags

    3xFLAG, GST, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-preSufI(IAC)

    Plasmid

    #168516

    Purpose

    Modified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    pre-SufI(IAC)
    

    Use

    Tags

    6xHisC tag and HSV tag

    Expression

    Bacterial

    Mutation

    C17I and C295A

    Promoter

    T7

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Stx3

    Plasmid

    #186685

    Purpose

    Expresses Syntaxin 3 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    Syntaxin 3
    

    Use

    Tags

    6 his tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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