We narrowed to 12,862 results for: LIC

1. pEF-pIgH-ESCargo(FTV,Orange)

   Plasmid

   #140161

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
   

   Use

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SLF060: Lenti-CaMKIIa-jRGECO1a

   Plasmid

   #112282

   Purpose

   For lentiviral or direct expression of cytosolic jRGECO1a in excitatory neurons

   Depositor

   Adam Cohen

   Article

   Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

   Insert

   jRGECO1a
   

   Use

   Lentiviral

   Tags

   NES-6xHIS

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIa

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Htb2-GFP

   Plasmid

   #117669

   Purpose

   Colorize the nuclei

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   Htb2-GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains the gene encoding the Histone Htb2 from …

   Promoter

   pGAP

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHBintN

   Plasmid

   #48135

   Purpose

   Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vector

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ki67∆repeats-mNeonGreen-IRESpuro2

   Plasmid

   #183742

   Purpose

   Expresses Ki67∆repeats-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67(lacking repeats) (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking repeats)

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. YIplac211-VPS8-HaloTagx2

   Plasmid

   #177113

   Purpose

   Expresses Vps8-HaloTagx2 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   VPS8 (VPS8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKar2-msGFP-HDEL

   Plasmid

   #117668

   Purpose

   Localisation of Endoplasmic Reticulum

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pKar2-msGFP-HDEL
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Contains the Kar2 promoter and signal sequence fr…

   Promoter

   pKar2

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTP443

   Plasmid

   #216361

   Purpose

   xNup98-Nb1t (+1Cys) Nanobody production

   Depositor

   Dirk Görlich

   Article

   Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

   Insert

   xNup98-Nb1t (+1Cys)
   

   Use

   Affinity Reagent/ Antibody

   Tags

   14xHis-NEDD8

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-NHX1-HaloTagx2

   Plasmid

   #177107

   Purpose

   Expresses Nhx1-HaloTagx2 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   NHX1 (NHX1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPDS1-mEGFP-IRES-puro2b

    Plasmid

    #26464

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    pPDS1 (PTTG1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAurora

    Plasmid

    #190158

    Purpose

    Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

    Type

    Empty backbone

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-Sport6 Baf60b

    Plasmid

    #24723

    Depositor

    Benoit Bruneau

    Article

    Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

    Insert

    Baf60b (Smarcd2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pETa(+) HpCsd3 (42-403)

    Plasmid

    #71285

    Purpose

    Expresses Csd3 from Helicobacter pylori

    Depositor

    SeWon Suh

    Article

    An et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    HP0506 (HP0506 )
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pZA-GFP-R5

    Plasmid

    #106256

    Purpose

    Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide

    Depositor

    Urartu Seker

    Article

    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Added silaffin R5 peptide to C terminus of GFP af…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAL1

    Plasmid

    #197285

    Purpose

    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-U1C

    Plasmid

    #1840

    Depositor

    Philip Leder

    Article

    Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

    Insert

    U1C (SNRPC Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

17. NODALvar-CAG-AAVS1

    Plasmid

    #115262

    Purpose

    For stable integration and expression (under control of the CAG promoter) of a human NODAL splice variant from the AAVS1 safe harbour locus in human cells

    Depositor

    Lynne Postovit

    Article

    Findlay et al (2018) bioRxiv 276170

    Insert

    NODAL (NODAL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZA-mCherry-R5

    Plasmid

    #106258

    Purpose

    Expresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetO

    Depositor

    Urartu Seker

    Article

    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

    Insert

    mCherry
    

    Use

    Tags

    Added silaffin R5 peptide to C terminus of mCherr…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

19. H2B-2A-GFP-IRESpuro2

    Plasmid

    #183746

    Purpose

    Expresses H2B-2A-GFP

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Use

    Tags

    GFP with 2A protease cleavage site between H2B an…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid

    #164266

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6 promoter from W05B2.8

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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