We narrowed to 7,128 results for: Trac;

1. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

   Plasmid

   #159803

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMEH305 HA NLS SpCas9 3x high affinity MS2 loops

   Plasmid

   #168216

   Purpose

   Mammalian expression SV40 and nucleoplasmin NLS SpCas9 with 3 high affinity MS2 loops and fused to an N-terminal HA tag

   Depositor

   Joshua Leonard

   Article

   Hung et al J Extracell Vesicles. 2016 May 13;5:3102

   Insert

   SV40 NLS SpCas9
   

   Use

   Lentiviral

   Tags

   HA, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.hSyanpsin.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209663

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   S29W.A95K

   Promoter

   hSyn

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBC155

   Plasmid

   #202274

   Purpose

   Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

   Depositor

   Paul Schulze-Lefert

   Article

   Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

   Insert

   bc182
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSyn-jRex-GECO

   Plasmid

   #169259

   Purpose

   AAV transfer plasmid for neuronal expression of jRex-GECO.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   JRex-GECO
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDestTol2CG2--3.5ubb:plin2-tdtomato

   Plasmid

   #172534

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with cmlc2:eGFP as a transgenesis marker

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAV103

   Plasmid

   #63193

   Purpose

   pRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Worm and Yeast

   Mutation

   Promoter

   Available since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-GCaMP3fast

   Plasmid

   #74422

   Purpose

   Express fluorescent calcium indicator GCaMP3fast in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2015 Nov 3;5:15978. doi: 10.103

   Insert

   GCaMP3fast
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W4Y D356A

   Promoter

   CMV

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. RFP-PASS

   Plasmid

   #193971

   Purpose

   Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

   Depositor

   Guangwei Du

   Article

   Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

   Insert

   PA biosenor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #112922

    Purpose

    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-UMI

    Plasmid

    #222694

    Purpose

    CRISPR-screening

    Depositor

    Ulrich Elling

    Article

    Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PGK without BsmBI cutsite

    Promoter

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEM246 - MCS mosTI unc-119 target

    Plasmid

    #159821

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    MCS mosTI unc-119 target
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEmerald-MyosinIIA-N-14

    Plasmid

    #54191

    Purpose

    Localization: Cytoskeleton, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Burnette et al J Cell Biol. 2014 Apr 14;205(1):83-96. d

    Insert

    MyosinIIA
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pcDNA3 GRK2 S670D

    Plasmid

    #45756

    Depositor

    Robert Lefkowitz

    Article

    Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

    Insert

    GRK2 S670D (GRK2 Bovine)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S670D

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-NPY-ClopHensor

    Plasmid

    #25939

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
    

    Use

    Tags

    NPY and Strep-II

    Expression

    Mammalian

    Mutation

    Neuropeptide Y localization signal

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

    Plasmid

    #159849

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-RiboL1-jGCaMP8f

    Plasmid

    #192612

    Purpose

    AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMuLE_ENTR_TOP-NLuc1.1_L5-L4

    Plasmid

    #113867

    Purpose

    Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Inserts

    TOP (wnt dependent promoter)
    NLuc
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. peCALWY-1

    Plasmid

    #22233

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-1 Zn(II) sensor (ATOX1 GFP's, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    linker consists of 9 GGSGGS repeats

    Promoter

    Available since

    Nov. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

    Plasmid

    #182351

    Purpose

    Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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