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pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Publication

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK1

Plasmid

#23498

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK1 (MAPK1 Human)

Use

Gateway Cloning

Mutation

missing first codon

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
Pdx1-RFP puro (DM#235)

Plasmid

#15085

Depositor

Douglas Melton

Publication

Rajagopal et al Science. 2003 Jan 17. 299(5605):363.

Insert

Pdx1 promoter (Pdx1 Mouse)

Tags

RFP

Expression

Bacterial

Available since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Publication

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-TRPM7

Plasmid

#23476

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRPM7 (TRPM7 Human)

Use

Gateway Cloning

Mutation

Insert encodes NCBI: NR_149154.2 bases 265 to 146…

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pHel2

Plasmid

#102960

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Publication

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Plasmid shuttle vector system for genetic complem…

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pIVM_26

Plasmid

#204501

Purpose

Expression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)

Depositor

Alessio Ciulli

Publication

Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

Insert

EloB (1-104) and EloC (17-112) (ELOC, ELOB Human)

Expression

Bacterial

Mutation

0

Promoter

AmpR

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLV-tetO-Cdx2

Plasmid

#70697

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Cdx2

Depositor

Hubert Schorle

Publication

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Cdx2 (Cdx2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTet-O-Nd1-puro

Plasmid

#52052

Purpose

Expresses Nd1 and puromycin resistent gene under control of TetON promoter

Depositor

Marius Wernig

Publication

Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral

Tags

T2A-and puromycin resistant cassette

Expression

Mammalian

Promoter

tet-on

Available since

April 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-ADRBK1

Plasmid

#23835

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ADRBK1 (GRK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pTTCC

Plasmid

#164264

Purpose

Expresses a thermostable Cas9 enzyme and gRNA in Thermus thermophilus

Depositor

Gudmundur Hreggvidsson

Publication

Adalsteinsson et al Sci Rep. 2021 May 5;11(1):9586. doi: 10.

Inserts

Cas9
gRNA scaffold

Expression

Bacterial

Promoter

Pcel and trc

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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pFG4

Plasmid

#87251

Purpose

gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

Depositor

Kerstin Bystricky

Publication

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Hyg

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Publication

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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betaRBPIG

Plasmid

#16422

Depositor

Juergen Hescheler

Publication

Pfannkuche et al Nat Protoc. 2008. 3(9):1510-26. doi: htt

Insert

loxP flanked RFP and blasticidin-resistance plus puromycin-acetyltransferase IRES GFP

Expression

Mammalian

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pTYB3-PUM1-HD

Plasmid

#17543

Depositor

Traci Hall

Publication

Wang et al Mol Cell. 2001 Apr . 7(4):855-65.

Insert

human PUM1 Pumilio homology domain (PUM1 Human)

Tags

intein

Expression

Bacterial

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRK5-myc-Miro1 P13V

Plasmid

#47889

Purpose

Expresses myc tagged Miro1 P13V constitutively active mutant

Depositor

Pontus Aspenström

Publication

Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

Insert

Miro1 P13V (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

P13V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRSETa_ mEos4a

Plasmid

#51072

Purpose

Photoconverible Fluorescent Protein_ Fixative Resistant

Depositor

Loren Looger

Publication

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

mEos4a

Expression

Bacterial

Promoter

T7

Available since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMDC123SB-AtTAS1c-B/c

Plasmid

#51772

Purpose

Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

Depositor

James Carrington

Publication

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtTAS1c-B/c

Use

Plant expression

Mutation

A. thaliana TAS1c precursor sequence including a …

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-MAP2K1

Plasmid

#23406

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Publication

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Tags

His6 followed by a thrombin site

Expression

Bacterial

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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