We narrowed to 1,249 results for: vent

1. pJK642

   Plasmid

   #72391

   Purpose

   Produces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)

   Depositor

   T. J. Kappock

   Insert

   glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Mutated tyrosine-397 to phenylalanine to block ad…

   Promoter

   T7

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK650

   Plasmid

   #72400

   Purpose

   Produces Escherichia coli glutamine synthetase with Y397F mutation that prevents adenylation (EcGlnA-Y397F)

   Depositor

   T. J. Kappock

   Insert

   glutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutated tyrosine-397 to phenylalanine to block ad…

   Promoter

   T7

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. VCB1-CKKp

   Plasmid

   #24597

   Depositor

   Kevin Truong

   Article

   Mills et al Protein Sci. 2009 Jun . 18(6):1252-60.

   Insert

   CB1-CKKp
   

   Use

   Tags

   Venus

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pSEVA63_sgRNA-B1_M13ps

   Plasmid

   #231422

   Purpose

   M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-B1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA26_sgRNA-2_M13ps

   Plasmid

   #231412

   Purpose

   M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEVA26_sgRNA-A1_M13ps

   Plasmid

   #231414

   Purpose

   M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   sgRNA-A1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBP-MS2

   Plasmid

   #65104

   Purpose

   can be used to isolate RNA binding complexes

   Depositor

   Josep Vilardell

   Article

   Macias et al Mol Cell. 2008 Jun 20;30(6):732-42. doi:

   Insert

   MS2
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   double mutation (V75Q and A81G) that prevents oli…

   Promoter

   tac

   Available since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLB

   Plasmid

   #11619

   Purpose

   pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

   Depositor

   Stephan Kissler

   Article

   Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

   Type

   Empty backbone

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEVA47_Helper

   Plasmid

   #231420

   Purpose

   M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

   Depositor

   Alfonso Jaramillo

   Article

   Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

   Insert

   gp1-gp11
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-CMV-polysite

    Plasmid

    #122843

    Purpose

    Gateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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