2,384 results
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Plasmid#182894PurposeMammalian expression of PVALB rat mAb HC geneDepositorInsertanti-PVALB (human) heavy chain (PVALB Rat)
UseAffinity Reagent/ AntibodyExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa
Plasmid#182895PurposeMammalian expression of PVALB rat mAb LC geneDepositorInsertanti-PVALB (human) light chain (PVALB Rat)
UseAffinity Reagent/ AntibodyExpressionMammalianMutationNoPromoterCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_C109A_Y107C
Plasmid#190174PurposeFor expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathwayDepositorInsertSLC6A4 Serotonin Transporter (Slc6a4 Rat)
TagsFLAG, His6, and c-mycExpressionMammalianMutationchanged Cys109 to alanine, changed tyr107 to cyst…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_rSERT_X5C_S277C
Plasmid#190177PurposeFor mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibilityDepositorInsertrat serotonin transporter (Slc6a4 Rat)
TagsFLAG, His6, and c-mycExpressionMammalianMutationCys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C1-3x-NLS-mScarlet-AMPKα2
Plasmid#192452PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.DepositorInsert3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
Tags3x Nuclear localization signal (NLS) and mScarletExpressionMammalianPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax
Plasmid#190154PurposeExpresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vectorDepositorInsertVamp3 (Vamp2 Rat)
UseAAVTagsP2AT2A-EGFP-caaxExpressionMammalianMutationTruncation that includes only rat Vamp3 AA #1-81PromoterMBPAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT +D122H
Plasmid#187426PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorExpressionInsectMutationchanged aspartic acid at 129 for histidinePromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAT +R111E
Plasmid#187425PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorExpressionInsectMutationchanged arginine at 118 to glutamic acidPromoterPH and p10Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b motor
Plasmid#187364PurposeExpresses rat myosin 1b isoform b motor domain labelled with EGFPDepositorAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1b-Tail
Plasmid#187365PurposeExpresses rat myosin 1b isoform b Tail domain labelled with EGFPDepositorAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Myo1-IQ-Tail
Plasmid#187363PurposeExpresses rat myosin 1b isoform b IQTail domain labelled with EGFPDepositorAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TetO(3G)-GCaMP6f-WPRE
Plasmid#186205PurposeExpress GCaMP6f under TetO(3G)DepositorInsertGCaMP6f
UseAAVTags6xHis (N terminal on insert), T7 epitope, and Xpr…PromoterTetO(3G)Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry-SynaptoZip
Plasmid#177318PurposeAAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoterDepositorInsertmCherry-SynaptoZip (Vamp2 Rat)
UseAAVTagsMyc and mCherryExpressionMammalianPromoterhSynAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα
Plasmid#183454PurposesgRNA targeting rat PKA-RIIα subunitsDepositorInsertsgRNA targeting rat PKA-RIIα (Prkar2a Rat)
UseLentiviralPromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-scrambled
Plasmid#183455PurposeThis control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα constructDepositorInsertscrambled sgRNA
UseLentiviralPromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97A-IRES-EGFP
Plasmid#183457PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97E-IRES-EGFP
Plasmid#183458PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_Tubb3 GFP(Y39TAG) KI
Plasmid#182678PurposeExpression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.DepositorExpressionMammalianPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
iLID-mRuby3-iTrkA
Plasmid#136510PurposeMammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)DepositorAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-mVenus-TrkC
Plasmid#182948PurposeTo express the rat TrkC fused in the N-terminus with mVenusDepositorInsertmVenus-TrkC (Ntrk3 Rat, Synthetic)
UseRetroviral and Synthetic BiologyTagsmVenusExpressionMammalianPromoterMMLVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only