We narrowed to 17,813 results for: She

1. pKK1 -pFlp-18::mito4x-GCaMP6f

   Plasmid

   #194969

   Purpose

   Fluorescent reporter of mitochondrial matrix Ca2+.

   Depositor

   Frederic Hoerndli

   Insert

   4 repeats of Cox8 presequence plus GCaMP6f
   

   Expression

   Worm

   Promoter

   Flp-18

   Available Since

   June 21, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pUltra-Smurf-luc-RTTA3

   Plasmid

   #59438

   Purpose

   silence doxycyclin inducible pUltra-dox (also expresses luciferase)

   Depositor

   Yildirim Dogan, Kitai Kim

   Inserts

   luciferase
   Reverse TET-Transactivator 3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   loxP5171

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2-RSV-jGCaMP7s-W

   Plasmid

   #126690

   Purpose

   Mammalian calcium sensor protein.

   Depositor

   Darrell Kotton

   Insert

   jGCaMP7s
   

   Use

   Lentiviral

   Tags

   6xHIS, T7, Xpress

   Mutation

   A52V, A317L

   Promoter

   RSV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sCFP in pBSDONR P4r-P2

   Plasmid

   #121530

   Purpose

   For making C-terminal sCFP fusions using multisite Gateway cloning

   Depositor

   Roger Innes

   Insert

   sCFP
   

   Expression

   Bacterial

   Available Since

   Aug. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mTFP1-mKO-FRET-8

   Plasmid

   #58152

   Purpose

   Localization: Cytosolic FRET, Excitation: 462 / 548, Emission: 492 / 561

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   mKO and mTFP1

   Expression

   Mammalian

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV Tor1a-V5

   Plasmid

   #175135

   Purpose

   Lentiviral expression of Tor1a-Myc

   Depositor

   Larry Gerace

   Insert

   Tor1a (Tor1a Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBluescript mouse ptc

   Plasmid

   #58699

   Purpose

   full length mouse ptc cDNA

   Depositor

   Matthew Scott

   Insert

   ptc (Ptch1 Mouse)
   

   Use

   Cloning vector

   Promoter

   T7

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pHAGE2-RSV-jGCaMP7f-W

   Plasmid

   #126691

   Purpose

   Mammalian calcium sensor protein.

   Depositor

   Darrell Kotton

   Insert

   jGCaMP7f
   

   Use

   Lentiviral

   Tags

   6xHIS, T7, Xpress

   Mutation

   H78K, A317L

   Promoter

   RSV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC6-TEFkFLP(LYS2)

   Plasmid

   #49458

   Purpose

   overespression of Flp step-arrest mutant in yeast, LYS2 selection

   Depositor

   Marc Gartenberg

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   TEF1

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid

    #171937

    Purpose

    Expresses APEX2 linked to tau for labeling microtubules

    Depositor

    David Berson

    Insert

    tau-APEX2
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    hsyn

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK266

    Plasmid

    #73326

    Purpose

    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    violacein biosynthesis protein VioD
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIC81

    Plasmid

    #63065

    Purpose

    Mammalian expression of EGFP-TEV-S-tag PMFI for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Insert

    PMFI, IMAGE clone 6148250 (PMF1 Human)
    

    Tags

    EYFP and TEV-S

    Expression

    Mammalian

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Dronpa-Peroxisomes-2

    Plasmid

    #54690

    Purpose

    Localization: Peroxisomes, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Insert

    Dronpa-Peroxisomes
    

    Tags

    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

14. PB CAG-L-bpA-SV40pA-L-td-sfGFP

    Plasmid

    #133575

    Purpose

    CAG tdGFP cre/lox reporter piggyBac transposon (bpA 1x SV40pA stop)

    Depositor

    Mario Capecchi

    Insert

    Tandem dimer superfolder GFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PTEN-VC

    Plasmid

    #89332

    Purpose

    expresses hPTEN fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Insert

    PTEN1 (PTEN Human)
    

    Tags

    C-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mRuby-Peroxisomes-2

    Plasmid

    #54840

    Purpose

    Localization: Peroxisomes, Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    mRuby-Peroxisomes
    

    Tags

    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

    Expression

    Mammalian

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pAAV-CAG-FLEX-splitTVA-EGFP

    Plasmid

    #59332

    Purpose

    Expresses splitTVA-P2A-EGFP

    Depositor

    Ian Wickersham

    Insert

    splitTVA950-P2A-EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B0030m_BC

    Plasmid

    #74394

    Purpose

    MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0030 to adjust spacing in MC system. [B:B0030m:C]

    Depositor

    Douglas Densmore

    Insert

    RBS
    

    Use

    Synthetic Biology

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK690

    Plasmid

    #72380

    Purpose

    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)

    Depositor

    T. J. Kappock

    Insert

    glutaredoxin-4
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H2B CFP

    Plasmid

    #198059

    Purpose

    mCerulean fused to human H2B protein

    Depositor

    Amro Hamdoun

    Insert

    H2B
    

    Use

    Synthetic Biology; Sea urchin, mammal, zebrafish

    Tags

    mCerulean

    Promoter

    sp6

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MB121 (Nf2 BBA)

    Plasmid

    #12170

    Depositor

    Tyler Jacks

    Insert

    Nf2 BBA (Nf2 Mouse)
    

    Expression

    Mammalian

    Mutation

    BBA: dominant negative Nf2, 7 Blue Box residues c…

    Available Since

    July 7, 2006

    Availability

    Academic Institutions and Nonprofits only

22. MAC045-C12

    Plasmid

    #58753

    Purpose

    bacterial expression of PDB 4NXJ

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4NXJ
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    Aug. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pJK409

    Plasmid

    #72423

    Purpose

    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK405

    Plasmid

    #72418

    Purpose

    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK403

    Plasmid

    #72416

    Purpose

    Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag

    Depositor

    T. J. Kappock

    Insert

    arylesterase
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. KBB610

    Plasmid

    #185111

    Purpose

    Pom152 full repeat domain in pEX-N-His for bacterial expression and purification

    Depositor

    Ken Belanger

    Insert

    POM152
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Pom152 Repeats-6His

    Available Since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PS-CFP2-mCherry-FRET-10

    Plasmid

    #58157

    Purpose

    Localization: Cytosolic FRET, Excitation: 490 / 587, Emission: 511 / 610

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    PS-CFP2 and mCherry

    Expression

    Mammalian

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

28. 371-Bc

    Plasmid

    #97028

    Purpose

    yORF-tev-His6

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    His6 tag and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 371-B

    Plasmid

    #97027

    Purpose

    His6-tev-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MIGR1-PREP1

    Plasmid

    #78147

    Purpose

    retroviral expression of PREP1 and GFP

    Depositor

    Francesco Blasi

    Insert

    PREP1 (PKNOX1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Available Since

    June 10, 2016

    Availability

    Academic Institutions and Nonprofits only

31. pHH0103 SMURF2 WW domain #3

    Plasmid

    #104226

    Purpose

    Bacterial expression of WW domain #3 from SMURF2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    SMURF2 WW domain #3 (SMURF2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHH0103 SMURF2 WW domain #2

    Plasmid

    #104225

    Purpose

    Bacterial expression of WW domain #2 from SMURF2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    SMURF2 WW domain #2 (SMURF2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHH0103 SMURF2 WW domain #1

    Plasmid

    #104224

    Purpose

    Bacterial expression of WW domain #1 from SMURF2

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    SMURF2 WW domain #1 (SMURF2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-synP-FLEX-EGFP-B19G

    Plasmid

    #59333

    Purpose

    Expresses EGFP-P2A-B19G

    Depositor

    Ian Wickersham

    Insert

    EGFP-P2A-B19G
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Synapsin-1

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

    Plasmid

    #137859

    Purpose

    Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pE-SUMO CRYGB

    Plasmid

    #80751

    Purpose

    Expression of human gamma B-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGB (CRYGB Human)
    

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mKO2-Peroxisomes-2

    Plasmid

    #54769

    Purpose

    Localization: Peroxisomes, Excitation: 551, Emission: 565

    Depositor

    Michael Davidson

    Insert

    mKO2-Peroxisomes
    

    Tags

    Peroxisomal Targeting Signal 1 (PTS1; Ser-Lys-Leu…

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pCB94

    Plasmid

    #69743

    Purpose

    Retroviral expression of PP2A beta

    Depositor

    Iain Cheeseman

    Insert

    PP2A beta (PTPA Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pJK365

    Plasmid

    #72404

    Purpose

    Produces Acetobacter aceti 1023 Est01

    Depositor

    T. J. Kappock

    Insert

    acyl-CoA thioester hydrolase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 4I79

    Plasmid

    #51319

    Depositor

    Cheryl Arrowsmith

    Insert

    4I79 (NUP43 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits