Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 165,385 results for: Gene

Showing: 2101 - 2120 of 165385 results

  2. pDONR221-HMGN1

    Plasmid
    #192718
    Purpose
    Gateway entry vector encoding human HMGN1
    Depositor
    Summer Thyme
    Insert
    HMGN1 (HMGN1 Human)
    Use
    Gateway entry vector
    Promoter
    None
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192718-plasmid-map-sequence-id-377319

    Enlarge

  3. pDTA-5

    Plasmid
    #128845
    Purpose
    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128845-plasmid-map-sequence-id-252002

    Enlarge

  5. pXP420 v2 (repaired Amp promoter)

    Plasmid
    #86920
    Purpose
    Shuttle vector to facilitate gene expression for metabolic engineering in S. cerevisiae. Contains TEF1 promoter and CYC1 terminator for insertion of gene of interest. Contains HIS3 selection marker.
    Depositor
    Gary Yellen
    Type
    Empty backbone
    Use
    Cre/Lox
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Feb. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86920-plasmid-map-sequence-id-172992

    Enlarge

  7. pZP05

    Plasmid
    #232319
    Purpose
    pZP05 was constructed by cloning the oriFn-repA fragment from pCWU6 into the smaller suicide plasmid pCM-galK, facilitating replication in Fusobacterium nucleatum
    Depositor
    Chenggang Wu
    Insert
    Ori-repA
    Expression
    Bacterial
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232319-plasmid-map-sequence-id-462197

    Enlarge

  8. YIplac211-Kar2-moxGFP2-HDEL

    Plasmid
    #218970
    Purpose
    Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2
    Depositor
    Benjamin Glick
    Insert
    KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
    Use
    Gene replacement vector for yeast
    Tags
    moxGFP2
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218970-plasmid-map-sequence-id-445666

    Enlarge

  9. pDONR221-nrip1b

    Plasmid
    #192732
    Purpose
    Gateway entry vector encoding zebrafish nrip1b
    Depositor
    Summer Thyme
    Insert
    nrip1b (nrip1b Zebrafish)
    Use
    Gateway entry vector
    Mutation
    L661P (rs511527097); S720P; A801T; K862E; A863P; …
    Promoter
    None
    Available Since
    Nov. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192732-plasmid-map-sequence-id-377800

    Enlarge

  10. pJK561

    Plasmid
    #72283
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin with Cys35>Ser mutant (AaTrxA-C35S)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Expression
    Bacterial
    Mutation
    changes cysteine-35 to serine
    Promoter
    T7
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72283-plasmid-map-sequence-id-131874

    Enlarge

  11. pJK523

    Plasmid
    #72442
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available Since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72442-plasmid-map-sequence-id-133863

    Enlarge

  12. pJK590

    Plasmid
    #71698
    Purpose
    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag (AaTrxB1H6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase 1
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71698-plasmid-map-sequence-id-131731

    Enlarge

  13. pCAGGS-PHFF-sh-hBVRA

    Plasmid
    #100285
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available Since
    Sept. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    100285-plasmid-map-sequence-id-194439

    Enlarge

  14. AICSDP-49: MYL2-mEGFP

    Plasmid
    #114414
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114414-plasmid-map-sequence-id-220893

    Enlarge

  15. AICSDP-46: MYL7-mEGFP

    Plasmid
    #114413
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    114413-plasmid-map-sequence-id-250980

    Enlarge

  16. pCAGGS-PHFF-sh-mBVRA

    Plasmid
    #100286
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for mouse BVRA gene in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Inserts
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for mouse BVRA
    Tags
    FLAG, T7 and Mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    All genes are codon-optimized for expression in h…
    Promoter
    CAG promoter and H1 promoter
    Available Since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100286-plasmid-map-sequence-id-194443

    Enlarge

  17. iMb-Notch-Mosaic (IR99.40)

    Plasmid
    #99749
    Purpose
    Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes
    Depositor
    Rui Benedito
    Article
    Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do
    Insert
    MbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST
    Use
    Cre/Lox and Mouse Targeting
    Expression
    Mammalian
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    99749-plasmid-map-sequence-id-199304

    Enlarge

  18. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid
    #60600
    Purpose
    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.
    Depositor
    Akitsu Hotta
    Article
    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22
    Inserts
    CRISPR Cas9 D10A
    puromycin resistance gene
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Changed Asp 10 to Ala, Codon usage optimized for …
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    60600-plasmid-map-sequence-id-97110

    Enlarge

  19. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

    Enlarge

  20. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130928-plasmid-map-sequence-id-257360

    Enlarge

Showing: 2101 - 2120 of 165385 results